HPLC Column, Amide, 4um, 4.6mm ID x 150mm Length, 100A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40036‑15P Amide HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, providing excellent selectivity, faster equilibration, and robust column lifetime for HILIC separations. The amide stationary phase delivers strong and reproducible interaction with amine‑containing and highly polar analytes, while the silica‑hydride backbone improves retention stability and reduces moisture‑layer variability compared to conventional amide‑bonded silica phases.
This column is optimized for laboratories operating standard analytical LC systems, including workflows using ELSD or Corona CAD detection, as well as UV‑absorbing, nitrogen‑containing compounds.
Application Focus: Amide Chemistry for HILIC Separations
Amide stationary phases are preferred for the analysis of highly polar analytes requiring stable HILIC retention. The Amide phase is particularly effective for:
Sugars and sugar alcohols
Amine‑containing compounds
Polar metabolites
Other HILIC‑appropriate polar analytes
The TYPE‑C™ silica hydride surface supports consistent retention and improved reproducibility, especially under high‑organic mobile‑phase conditions commonly used in HILIC.
Key Advantages of Cogent TYPE‑C™ Amide Technology
Compared to traditional amide HILIC columns, this column provides:
Faster equilibration between injections
Improved retention stability across mobile‑phase compositions
Robust column lifetime under demanding HILIC conditions
Reduced moisture sensitivity, improving run‑to‑run consistency
These characteristics make the Amide TYPE‑C™ column suitable for both routine analysis and advanced HILIC method development.
Why Choose the 4.6 mm × 150 mm Format
The 4.6 mm ID × 150 mm length configuration is a popular analytical format when enhanced resolution is required for complex polar mixtures while maintaining compatibility with standard LC systems:
Higher resolving power and peak capacity than 50 mm and 100 mm formats
Standard analytical flow rates compatible with most HPLC instruments
Excellent suitability for ELSD, Corona CAD, and UV detection
Ideal for nitrogen‑containing compounds with UV absorbance
This format is frequently selected when separation confidence is prioritized over the shortest possible run time.
Particle Size and Performance
The 4 µm particle size delivers a practical balance between efficiency, robustness, and manageable backpressure, resulting in reliable peak shape and reproducible performance on standard analytical HPLC systems.
System Requirements and Flow Rates
Typical flow range (4.6 mm ID): ~1.0–1.5 mL/min
System capability: Compatible with standard analytical HPLC systems
Note: No specialized low‑flow or UHPLC‑only equipment is required
Flow rates should be optimized based on mobile‑phase viscosity, column temperature, and system pressure limits.