Catalog Number - 40036-15P-2

The 40036‑15P‑2 Amide HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, providing excellent selectivity, faster equilibration, and robust column lifetime for HILIC separations. The amide stationary phase delivers strong and reproducible interaction with amine‑containing and highly polar analytes, while the silica‑hydride surface improves retention stability by minimizing moisture‑layer variability common to conventional amide‑bonded silica phases.

This column is optimized for high‑resolution analytical and LC‑MS workflows, where enhanced separation is required for complex polar mixtures.

Application Focus: Amide Chemistry for HILIC Separations

Amide stationary phases are specifically selected for highly polar analytes that require stable, reproducible HILIC retention. The Amide phase is particularly effective for:

• Sugars and sugar alcohols

• Amine‑containing compounds

• Polar metabolites

• Other HILIC‑appropriate polar analytes

The TYPE‑C™ silica hydride backbone supports consistent retention and improved reproducibility, especially under high‑organic mobile‑phase conditions.

Key Advantages of Cogent TYPE‑C™ Amide Technology

Compared to traditional amide HILIC columns, this column offers:

• Faster equilibration between gradient runs

• Improved retention stability across varying mobile‑phase compositions

• Robust column lifetime under demanding HILIC conditions

• Reduced water‑layer variability, improving run‑to‑run consistency

These features make the Amide TYPE‑C™ column suitable for both routine analysis and advanced HILIC method development.

Why Choose the 2.1 mm × 150 mm Format

The 2.1 mm ID × 150 mm length configuration is commonly selected when maximum analytical resolution is desired, particularly for LC‑MS applications:

• Higher resolving power than 50 mm and 100 mm formats

• Increased peak capacity for complex polar analytes

• Reduced solvent consumption compared to larger‑diameter columns

• Excellent LC‑MS compatibility, with lower flow to the MS source

This format is ideal when separation confidence and sensitivity are prioritized over shortest run time.

Particle Size and Performance

The 4 µm particle size provides a balance of efficiency, robustness, and manageable backpressure, delivering reliable peak shape and reproducible performance on modern analytical and LC‑MS‑capable HPLC systems.

System Requirements and Flow Rates

• Typical flow range (2.1 mm ID): ~0.2–0.3 mL/min

• System capability: Requires accurate, stable low‑flow delivery

• Important note: Not all standard HPLC systems are suitable for reliable operation at these reduced flow rates

Flow rates should be optimized based on mobile‑phase viscosity, column temperature, and system pressure limits.