CUSTOM ITEM: HPLC Column, Amide, 4um, 3.0mm ID x 250mm Length, 100A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40036‑25P‑3 Amide HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, delivering excellent selectivity, faster equilibration, and robust column lifetime for demanding HILIC separations. The amide stationary phase provides strong, reproducible interaction with amine‑containing and highly polar analytes, while the silica‑hydride surface minimizes moisture‑layer instability associated with conventional bonded‑silica amide phases.
This column is produced as a custom, made‑to‑order item and is specifically selected when extended retention or additional separation between closely related compounds is required.
Application Focus: Amide Chemistry for High‑Resolution HILIC Separations
Amide stationary phases are especially effective for highly polar analytes that benefit from stable, reproducible HILIC retention. The Amide phase is commonly chosen for:
Sugars and sugar alcohols
Amine‑containing compounds
Polar metabolites
Other HILIC‑appropriate polar analytes
The TYPE‑C™ silica hydride backbone supports consistent retention and improved reproducibility, particularly under high‑organic mobile‑phase conditions where conventional amide silica phases may show variability
Key Advantages of Cogent TYPE‑C™ Amide Technology
Compared to conventional amide HILIC columns, this column provides:
Faster equilibration between injections
Improved retention stability across mobile‑phase compositions
Robust column lifetime under extended HILIC gradients
Reduced moisture sensitivity, improving run‑to‑run consistency
These advantages make the Amide TYPE‑C™ column well suited for applications requiring maximum separation and method robustness.
Why Choose the 3.0 mm × 250 mm Format
The 3.0 mm ID × 250 mm length configuration is selected when maximum resolving power and extended retention are required:
Greater separation of closely related polar compounds
Run times similar to 4.6 mm ID columns, but with lower solvent use
Reduced operating flow rates, decreasing mobile‑phase consumption
No specialized low‑flow hardware required, compatible with standard analytical HPLC systems
This solvent‑efficient format is ideal when long columns are required but laboratories prefer to avoid the higher solvent costs of 4.6 mm ID formats.
Particle Size and Performance
The 4 µm particle size provides an effective balance of efficiency, robustness, and manageable backpressure, enabling reliable high‑resolution separations on standard analytical HPLC platforms.
System Requirements and Flow Rates
Typical flow range (3.0 mm ID): ~0.4–0.6 mL/min
System capability: Compatible with standard analytical HPLC systems
Note: No specialized low‑flow or UHPLC‑only equipment is required
Flow rates should be optimized based on mobile‑phase viscosity, column temperature, and system pressure limits.
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable