Prep HPLC Column, Amide, 4um, 100A. 21.2mm ID x 100mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40036‑P21‑100 Amide Preparative HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, delivering excellent selectivity, faster equilibration, and robust column lifetime for preparative HILIC separations. The amide stationary phase provides strong, reproducible interaction with amine‑containing and highly polar analytes, while the silica‑hydride surface minimizes moisture‑layer effects that can destabilize retention on conventional bonded‑silica amide phases.
This column is designed for full preparative purification workflows where higher resolving power than short preparative formats is required while maintaining efficient throughput.
Preparative Use Case: Amide Chemistry for HILIC Purification
Amide stationary phases are particularly effective for preparative purification (“prep”) of highly polar compounds that require stable and reproducible HILIC retention. This column is commonly selected for isolation of:
Sugars and sugar alcohols
Amine‑containing analytes
Polar metabolites
Other HILIC‑appropriate polar compounds
The TYPE‑C™ amide phase supports rapid re‑equilibration and consistent retention, improving recovery and repeatability in preparative environments.
Why Choose the 21.2 mm × 100 mm Preparative Format
The 21.2 mm ID × 100 mm length configuration provides a balance between throughput and improved resolution compared to 50 mm preparative columns:
Higher resolving power than 21.2 × 50 mm formats
Larger sample loading capacity and fraction volumes than semi‑prep columns
Improved separation of closely eluting polar analytes
Suitable when purity requirements exceed what short prep columns can deliver, without the longer run times of 150 mm or 250 mm formats
This format is often selected when analytical separations are established and scale‑up requires additional separation confidence.
Key Advantages of Cogent TYPE‑C™ Amide Technology
Compared to conventional amide silica phases, this column provides:
Faster equilibration between preparative runs
Improved retention stability under HILIC conditions
Robust column lifetime during extended high‑organic operation
Reduced moisture sensitivity, improving reproducibility across batches
These benefits are especially valuable for preparative workflows where yield, purity, and repeatability are critical.
Particle Size and Performance
The 4 µm particle size offers a practical balance between efficiency and permeability, providing reliable preparative separations with manageable backpressure while maintaining strong recovery during fraction collection.
System Requirements
Typical operating flow range (21.2 mm ID): ~10–30 mL/min (method dependent)
Requires a preparative HPLC system capable of:
Sustained high‑flow delivery
Large injection volumes
Fraction‑collection capability
Analytical and semi‑preparative HPLC systems are not suitable for 21.2 mm ID preparative columns.
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable