Prep HPLC Column, Amide, 4um, 100A. 21.2mm ID x 150mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40036‑P21‑150 Amide Preparative HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, delivering excellent selectivity, faster equilibration, and robust column lifetime for high‑resolution preparative HILIC separations. The amide stationary phase provides strong, reproducible interaction with amine‑containing and highly polar analytes, while the silica‑hydride surface minimizes moisture‑layer variability that can compromise retention stability on conventional bonded‑silica amide phases.
This column is designed for full preparative purification workflows where additional resolving power is required beyond shorter preparative formats, while maintaining efficient recovery at elevated loadings.
Preparative Use Case: Amide Chemistry for High‑Resolution HILIC Purification
Amide stationary phases are particularly effective for preparative purification (“prep”) of highly polar compounds that require stable and reproducible HILIC retention. This column is commonly selected for isolation of:
Sugars and sugar alcohols
Amine‑containing analytes
Polar metabolites
Other HILIC‑appropriate polar compounds
The TYPE‑C™ amide phase supports consistent retention and rapid re‑equilibration, improving yield, purity, and repeatability in preparative environments.
Why Choose the 21.2 mm × 150 mm Preparative Format
The 21.2 mm ID × 150 mm length configuration is selected when greater resolving power and peak capacity are required for closely eluting polar compounds:
Higher resolution than 21.2 × 50 mm and 21.2 × 100 mm formats
Improved separation of structurally similar analytes
Large sample loading capacity with efficient fraction collection
Suitable when purity requirements exceed what mid‑length prep columns can deliver, but maximum 250 mm resolution is not required
This format provides a strong balance between resolution, throughput, and run time for demanding preparative HILIC workflows.
Key Advantages of Cogent TYPE‑C™ Amide Technology
Compared to conventional amide silica phases, this column provides:
Faster equilibration between preparative runs
Improved retention stability under HILIC conditions
Robust column lifetime during extended high‑organic operation
Reduced moisture sensitivity, improving batch‑to‑batch reproducibility
These advantages are especially valuable in preparative settings where consistency, purity, and yield are critical.
Particle Size and Performance
The 4 µm particle size offers a practical balance between efficiency and permeability, enabling reliable high‑resolution separations with manageable backpressure while maintaining strong recovery during fraction collection.
System Requirements
Typical operating flow range (21.2 mm ID): ~10–30 mL/min (method dependent)
Requires a preparative HPLC system capable of:
Sustained high‑flow delivery
Large injection volumes
Fraction‑collection capability
Analytical and semi‑preparative HPLC systems are not suitable for 21.2 mm ID preparative columns.
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable