Prep HPLC Column, Amide, 4um, 100A. 21.2mm ID x 250mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40036‑P21‑250 Amide Preparative HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, delivering excellent selectivity, faster equilibration, and robust column lifetime for maximum‑resolution preparative HILIC separations. The amide stationary phase provides strong, reproducible interaction with amine‑containing and highly polar analytes, while the silica‑hydride surface minimizes moisture‑layer instability that can compromise retention on conventional bonded‑silica amide phases.
This column is designed for full‑scale preparative purification where extended retention and the highest possible separation between closely related polar compounds are required.
Preparative Use Case: Amide Chemistry for Maximum‑Resolution HILIC Purification
Amide stationary phases are particularly effective for preparative purification (“prep”) of highly polar compounds that demand stable, reproducible HILIC retention. This column is commonly selected for preparative isolation of:
Sugars and sugar alcohols
Amine‑containing analytes
Polar metabolites
Other HILIC‑appropriate polar compounds requiring high‑purity isolation
The TYPE‑C™ amide phase supports consistent retention behavior and rapid re‑equilibration, enabling reliable, repeatable performance during extended preparative runs.
Why Choose the 21.2 mm × 250 mm Preparative Format
The 21.2 mm ID × 250 mm length configuration is selected when maximum resolving power and peak capacity are required in preparative workflows:
Highest resolution within the 21.2 mm Amide preparative family
Extended retention for closely related or structurally similar polar analytes
Excellent separation confidence when purity requirements are stringent
Ideal when longer run times are acceptable in exchange for superior purification
This format is typically chosen when shorter preparative columns (50 mm, 100 mm, or 150 mm) do not provide sufficient separation for demanding HILIC purifications.
Key Advantages of Cogent TYPE‑C™ Amide Technology
Compared to conventional amide silica phases, this column provides:
Faster equilibration between preparative runs
Improved retention stability under high‑organic HILIC conditions
Robust column lifetime during extended purification campaigns
Reduced moisture sensitivity, improving batch‑to‑batch reproducibility
These characteristics make the Amide TYPE‑C™ phase particularly well suited for high‑confidence, large‑scale preparative purification.
Particle Size and Performance
The 4 µm particle size delivers a practical balance of efficiency and permeability, enabling high‑resolution separations with manageable backpressure on preparative systems while maintaining strong recovery during fraction collection.
System Requirements
Typical operating flow range (21.2 mm ID): ~10–30 mL/min (method dependent)
Requires a preparative HPLC system capable of:
Sustained high‑flow delivery
Large injection volumes
Dedicated fraction‑collection capability
Analytical and semi‑preparative HPLC systems are not suitable for 21.2 mm ID preparative columns.
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable