Prep HPLC Column, Amide, 4um, 100A. 21.2mm ID x 50mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40036‑P21‑50 Amide Preparative HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, delivering excellent selectivity, faster equilibration, and robust column lifetime for preparative HILIC separations. The amide stationary phase provides strong, reproducible interaction with amine‑containing and highly polar analytes, while the silica‑hydride surface minimizes moisture‑layer variability that can impact conventional bonded‑silica amide phases.
This column is designed for full preparative purification, enabling high sample loading and efficient recovery when larger quantities of material must be isolated.
Preparative Use Case: Amide Chemistry for HILIC Purification
Amide stationary phases are particularly effective for purification (“prep”) of highly polar compounds requiring stable HILIC retention. This column is commonly selected for preparative isolation of:
Sugars and sugar alcohols
Amine‑containing analytes
Polar metabolites
Other HILIC‑appropriate polar compounds
The TYPE‑C™ amide phase supports reproducible retention and rapid re‑equilibration, improving throughput and reliability in preparative workflows.
Why Choose the 21.2 mm × 50 mm Preparative Format
The 21.2 mm ID × 50 mm length configuration represents a throughput‑focused preparative format, bridging analytical and larger‑scale purification:
Very high sample loading capacity and large fraction volumes
Fast run times due to the short 50 mm length
Efficient recovery when speed and yield are prioritized
Ideal when purity requirements are defined analytically and rapid isolation is needed
This format is frequently chosen when preparative throughput and turnaround time take priority over maximum resolving power.
Key Advantages of Cogent TYPE‑C™ Amide Technology
Compared to conventional amide silica phases, this column provides:
Faster equilibration between preparative runs
Improved retention stability under HILIC conditions
Robust column lifetime during repeated high‑organic operation
Reduced moisture sensitivity, improving reproducibility
These advantages are especially valuable in preparative environments where repeatability and yield are critical.
Particle Size and Performance
The 4 µm particle size offers a practical balance of efficiency and permeability, enabling reliable separations with manageable backpressure on preparative systems while maintaining strong recoveries during fraction collection.
System Requirements
Typical operating flow range (21.2 mm ID): ~10–30 mL/min (method dependent)
Requires a preparative HPLC system capable of:
Sustained high‑flow delivery
Large injection volumes
Fraction‑collection capability
Analytical and semi‑preparative HPLC systems are not suitable for 21.2 mm ID preparative columns.
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable