Prep HPLC Column, Amide, 4um, 100A. 30mm ID x 100mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40036‑P30‑100 Amide Preparative HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, delivering excellent selectivity, faster equilibration, and robust column lifetime for large‑scale preparative HILIC separations. The amide stationary phase provides strong, reproducible interaction with amine‑containing and highly polar analytes, while the silica‑hydride surface minimizes moisture‑layer variability that can compromise retention and reproducibility with conventional bonded‑silica amide phases.
This column is designed for high‑capacity preparative purification workflows where greater resolving power than short 30 mm formats is required while maintaining efficient throughput.
Preparative Use Case: Amide Chemistry for High‑Capacity HILIC Purification
Amide stationary phases are particularly effective for preparative purification (“prep”) of highly polar compounds that require stable, reproducible HILIC retention at elevated loadings. This column is commonly selected for purification of:
Sugars and sugar alcohols
Amine‑containing analytes
Polar metabolites
Other HILIC‑appropriate polar compounds requiring fraction collection
The TYPE‑C™ amide phase supports consistent retention and rapid re‑equilibration, improving yield, purity, and repeatability in large‑scale preparative workflows.
Why Choose the 30 mm × 100 mm Preparative Format
The 30 mm ID × 100 mm length configuration provides a balance between throughput and improved resolution compared to 30 × 50 mm preparative columns:
Higher resolving power than 30 × 50 mm formats
Improved separation of closely eluting polar analytes
Very high sample loading capacity with large fraction volumes
Suitable when purity requirements exceed what short prep columns can deliver, but maximum 150 mm or 250 mm resolution is not required
This format is often chosen when analytical separations are established and scale‑up demands additional separation confidence without excessively long run times.
Key Advantages of Cogent TYPE‑C™ Amide Technology
Compared to conventional amide silica phases, this column provides:
Faster equilibration between preparative runs
Improved retention stability under high‑organic HILIC conditions
Robust column lifetime during extended large‑scale operation
Reduced moisture sensitivity, improving batch‑to‑batch reproducibility
These advantages are especially valuable in preparative environments where consistency, yield, and throughput are critical.
Particle Size and Performance
The 4 µm particle size offers a practical balance between efficiency and permeability, enabling reliable high‑capacity separations with manageable backpressure on large‑scale preparative systems while maintaining strong recovery during fraction collection.
System Requirements
Typical operating flow range (30 mm ID): ~20–60 mL/min (method dependent)
Requires a large‑scale preparative HPLC system capable of:
Sustained high‑flow delivery
Large injection volumes
Dedicated fraction‑collection hardware
Analytical and semi‑preparative HPLC systems are not suitable for 30 mm ID preparative columns.
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable