Catalog Number - 40036-P30-250

The 40036‑P30‑250 Amide Preparative HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, delivering excellent selectivity, faster equilibration, and robust column lifetime for maximum‑resolution, large‑scale preparative HILIC separations. The amide stationary phase provides strong, reproducible interaction with amine‑containing and highly polar analytes, while the silica‑hydride surface minimizes moisture‑layer instability that often compromises retention consistency in conventional bonded‑silica amide phases.

This column is designed for full‑scale preparative purification when extended retention and the highest possible separation between closely related polar compounds are required.

Preparative Use Case: Amide Chemistry for Maximum‑Resolution HILIC Purification

Amide stationary phases are particularly effective for preparative purification (“prep”) of highly polar compounds that demand stable and reproducible HILIC retention across long gradients. This column is commonly selected for preparative isolation of:

• Sugars and sugar alcohols

• Amine‑containing analytes

• Polar metabolites

• Other HILIC‑appropriate polar compounds requiring high‑purity fraction collection

The TYPE‑C™ amide phase supports stable retention behavior and rapid re‑equilibration, ensuring repeatable performance during extended preparative runs.

Why Choose the 30 mm × 250 mm Preparative Format

The 30 mm ID × 250 mm length configuration is selected when maximum resolving power and peak capacity are required in large‑scale Amide HILIC workflows:

• Highest resolution within the 30 mm Amide preparative family

• Extended retention for closely related or structurally similar polar analytes

• Excellent separation confidence when purity requirements are stringent

• Ideal when longer run times are acceptable in exchange for superior purification

This format is typically chosen when 50 mm, 100 mm, or 150 mm preparative columns do not provide sufficient separation for demanding applications.

Key Advantages of Cogent TYPE‑C™ Amide Technology

Compared to conventional amide silica phases, this column provides:

• Faster equilibration between preparative runs

• Improved retention stability under high‑organic HILIC conditions

• Robust column lifetime during extended purification campaigns

• Reduced moisture sensitivity, improving batch‑to‑batch reproducibility

These attributes make the Amide TYPE‑C™ phase particularly well suited for high‑confidence, large‑scale preparative purification.

Particle Size and Performance

The 4 µm particle size delivers a reliable balance of efficiency and permeability, enabling high‑resolution separations with manageable backpressure on large‑scale preparative systems while maintaining strong recovery during fraction collection.

System Requirements

• Typical operating flow range (30 mm ID): ~20–60 mL/min (method dependent)

• Requires a large‑scale preparative HPLC system capable of:

  • Sustained high‑flow delivery

  • Large injection volumes

  • Dedicated fraction‑collection hardware

Analytical and semi‑preparative HPLC systems are not suitable for 30 mm ID preparative columns.

Made‑to‑Order Columns & Pricing Information

This is a custom, made‑to‑order column.

• Columns are manufactured specifically to order

• Orders cannot be canceled once placed

• Custom columns are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification: Class A Custom Item

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.