Catalog Number - 40036-P30-50

The 40036‑P30‑50 Amide Preparative HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, delivering excellent selectivity, faster equilibration, and robust column lifetime for large‑scale preparative HILIC separations. The amide stationary phase provides strong, reproducible interaction with amine‑containing and highly polar analytes, while the silica‑hydride surface minimizes moisture‑layer variability that can compromise retention and reproducibility on conventional bonded‑silica amide phases.

This column is designed for large‑bore preparative purification workflows where very high sample loading and rapid turnaround are required.

Preparative Use Case: Amide Chemistry for High‑Throughput HILIC Purification

Amide stationary phases are particularly effective for preparative purification (“prep”) of highly polar compounds that require stable HILIC retention at elevated loadings. This column is commonly selected for purification of:

• Sugars and sugar alcohols

• Amine‑containing analytes

• Polar metabolites

• Other HILIC‑appropriate polar compounds requiring fraction collection

The TYPE‑C™ amide phase enables consistent retention and fast re‑equilibration, supporting reliable, repeatable performance in production‑scale purification environments.

Why Choose the 30 mm × 50 mm Preparative Format

The 30 mm ID × 50 mm length configuration represents a throughput‑optimized large‑scale preparative format, scaling beyond 21.2 mm ID columns:

• Very high sample loading capacity and large fraction volumes

• Fast run times driven by the short 50 mm column length

• Efficient recovery when speed and yield are prioritized

• Ideal for workflows where analytical separation is already defined and rapid isolation is required

This format is frequently chosen when production throughput and turnaround take priority over maximum resolving power.

Key Advantages of Cogent TYPE‑C™ Amide Technology

Compared to conventional amide silica phases, this column provides:

• Faster equilibration between preparative runs

• Improved retention stability under high‑organic HILIC conditions

• Robust column lifetime during repeated large‑scale operation

• Reduced moisture sensitivity, improving batch‑to‑batch reproducibility

These advantages are especially valuable in preparative environments where consistency and yield are critical.

Particle Size and Performance

The 4 µm particle size offers a practical balance between efficiency and permeability, enabling reliable separations with manageable backpressure on large‑scale preparative systems while maintaining strong recovery during fraction collection.

System Requirements

• Typical operating flow range (30 mm ID): ~20–60 mL/min (method dependent)

• Requires a large‑scale preparative HPLC system capable of:

  • Sustained high‑flow delivery

  • Large injection volumes

  • Dedicated fraction‑collection hardware

Analytical and semi‑preparative HPLC systems are not suitable for 30 mm ID preparative columns.

Made‑to‑Order Columns & Pricing Information

This is a custom, made‑to‑order column.

• Columns are manufactured specifically to order

• Orders cannot be canceled once placed

• Custom columns are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification: Class A Custom Item

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.