Semi Prep HPLC Column, Amide, 4um, 100A. 10mm ID x 100mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40036‑SP100 Amide Semi‑Preparative HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, delivering excellent selectivity, faster equilibration, and robust column lifetime for HILIC‑mode purification. The amide stationary phase provides strong, reproducible interaction with amine‑containing and highly polar analytes, while the silica‑hydride surface reduces moisture‑related variability that can affect conventional bonded‑silica amide phases.
This column is designed for semi‑preparative purification workflows where material recovery and improved resolution are required beyond short, throughput‑focused configurations.
Semi‑Preparative Use Case: Amide Chemistry for HILIC Purification
Amide stationary phases are particularly effective for purification (“prep”) of highly polar compounds, making this column well suited when fractions are collected for downstream use. Typical semi‑prep applications include purification of:
Sugars and sugar alcohols
Amine‑containing analytes
Polar metabolites
Other HILIC‑appropriate polar compounds
The TYPE‑C™ amide phase provides stable retention and rapid re‑equilibration, improving reproducibility and throughput in semi‑preparative workflows.
Why Choose the 10 mm × 100 mm Semi‑Prep Format
The 10 mm ID × 100 mm length configuration offers a balance between throughput and increased resolving power compared to shorter semi‑prep columns:
Higher resolving power than 10 × 50 mm formats
Improved separation of closely eluting polar analytes
Higher sample loading than analytical columns
Efficient fraction collection without excessive run times
This format is commonly selected when analytical methods are established and additional resolution is needed during scale‑up.
Key Advantages of Cogent TYPE‑C™ Amide Technology
Compared to conventional amide silica phases, this column provides:
Faster equilibration between injections
Improved retention stability under HILIC conditions
Robust column lifetime in high‑organic mobile phases
Reduced moisture sensitivity, improving purification consistency
These advantages are especially valuable in semi‑preparative workflows where repeatability and yield are critical.
Particle Size and Performance
The 4 µm particle size balances efficiency and permeability, enabling reliable separations with manageable backpressure at semi‑preparative flow rates while maintaining good recovery during fraction collection.
System Requirements
Typical flow range (10 mm ID): ~3–8 mL/min (method dependent)
Requires a specialized semi‑preparative HPLC system capable of:
Higher flow delivery
Larger injection volumes
Fraction‑collection capability
Standard analytical HPLC systems are not suitable for 10 mm ID semi‑preparative columns.
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable