Semi Prep HPLC Column, Amide, 4um, 100A. 10mm ID x 250mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40036‑SP250 Amide Semi‑Preparative HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, delivering excellent selectivity, faster equilibration, and robust column lifetime for high‑resolution HILIC purification. The amide stationary phase provides strong and reproducible interaction with amine‑containing and highly polar analytes, while the silica‑hydride surface reduces moisture‑related variability often encountered with conventional bonded‑silica amide phases.
This column is designed for maximum‑resolution semi‑preparative workflows, where extended retention and greater separation between closely related polar compounds are required prior to fraction collection.
Semi‑Preparative Use Case: Amide Chemistry for High‑Resolution HILIC Purification
Amide stationary phases are particularly effective for purification (“prep”) of highly polar analytes when consistent HILIC retention is required at increased loading levels. This column is commonly selected for purification of:
Sugars and sugar alcohols
Amine‑containing analytes
Polar metabolites
Other HILIC‑appropriate polar compounds requiring fraction collection
The TYPE‑C™ amide phase offers stable retention and rapid re‑equilibration, supporting reproducible separations during extended semi‑preparative runs.
Why Choose the 10 mm × 250 mm Semi‑Prep Format
The 10 mm ID × 250 mm length configuration is selected when maximum resolving power and extended retention are needed in semi‑preparative applications:
Greatest separation between closely related polar analytes
Higher peak capacity than 10 × 50 mm and 10 × 100 mm formats
Increased sample loading compared to analytical columns
Ideal when purity and separation confidence are prioritized over shortest run time
This long semi‑prep format bridges analytical optimization and preparative scale‑up when difficult separations demand additional resolution.
Key Advantages of Cogent TYPE‑C™ Amide Technology
Compared to conventional amide silica phases, this column provides:
Faster equilibration between injections
Improved retention stability under high‑organic HILIC conditions
Robust column lifetime during extended purification sequences
Reduced moisture sensitivity, improving repeatability across runs
These advantages are particularly valuable in semi‑preparative purification workflows where consistency and yield are critical.
Particle Size and Performance
The 4 µm particle size provides an effective balance between efficiency and permeability, enabling high‑resolution separations with manageable backpressure at semi‑preparative flow rates while maintaining good recovery during fraction collection.
System Requirements
Typical flow range (10 mm ID): ~3–8 mL/min (method dependent)
Requires a specialized semi‑preparative HPLC system capable of:
Sustained higher flow delivery
Larger injection volumes
Fraction‑collection capability
Standard analytical HPLC systems are not suitable for 10 mm ID semi‑preparative columns.
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable