Catalog Number - 40036-SP50

The 40036‑SP50 Amide Semi‑Preparative HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, providing excellent selectivity, faster equilibration, and robust column lifetime for HILIC‑mode purification. The amide stationary phase delivers strong interaction with amine‑containing and highly polar analytes, while the silica‑hydride surface minimizes moisture‑related variability that can impact conventional bonded phases.

This column is designed for semi‑preparative purification workflows where material recovery is required rather than purely analytical measurement.

Semi‑Preparative Use Case: Amide Chemistry for HILIC Purification

Amide stationary phases are especially effective for purification (“prep”) of highly polar compounds, making this column well suited when fractions are collected for downstream use. Typical applications include purification of:

• Sugars and sugar alcohols

• Amine‑containing analytes

• Polar metabolites

• Other HILIC‑appropriate compounds requiring fraction collection

The TYPE‑C™ amide phase offers reproducible retention and fast re‑equilibration, improving throughput in semi‑preparative workflows.

Why Choose the 10 mm × 50 mm Semi‑Prep Format

The 10 mm ID × 50 mm length configuration represents a true semi‑preparative format, bridging analytical method development and full preparative purification:

• Higher sample loading capacity than analytical columns

• Rapid run times driven by the short 50 mm length

• Efficient fraction collection with manageable solvent volumes

• Ideal when speed and yield are prioritized over maximum resolution

This format is commonly selected when sufficient resolution has already been established analytically and scale‑up is required.

Key Advantages of Cogent TYPE‑C™ Amide Technology

Compared to conventional amide silica phases, this column provides:

• Faster equilibration between injections

• Improved retention stability under HILIC conditions

• Robust column lifetime in high‑organic mobile phases

• Reduced moisture sensitivity, supporting consistent purification performance

These advantages are particularly valuable in semi‑prep workflows where repeatability and throughput are critical.

Particle Size and Performance

The 4 µm particle size balances efficiency and permeability, providing reliable separation with manageable backpressure at semi‑preparative flow rates, while maintaining strong recoveries during fraction collection.

System Requirements

• Typical flow range (10 mm ID): ~3–8 mL/min (method dependent)

• Requires a specialized semi‑preparative HPLC system capable of:

  • Higher flow delivery

  • Larger injection volumes

  • Fraction collection capability

Standard analytical HPLC systems are not suitable for 10 mm ID semi‑preparative columns.

Made‑to‑Order Columns & Pricing Information

This is a custom, made‑to‑order column.

• Columns are manufactured specifically to order

• Orders cannot be canceled once placed

• Custom columns are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering assistance. Classification: Class A Custom Item

• This item is classified as a CUSTOM ITEM. View our Custom Item policy.