HPLC Column, Diol, 4um, 4.6mm ID x 150mm Length, 100A. Cogent TYPE-C Brand. 1 EA.

Additional Info:

The 40060‑15P Diol HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, providing excellent selectivity, faster equilibration, and robust column lifetime compared with conventional bonded‑silica diol columns. The 4 µm silica hydride particles deliver consistent chromatographic performance at moderate backpressure, making this column well suited for extended analytical runs and routine method development.

The TYPE‑C™ silica hydride surface supports stable, reproducible retention behavior across reversed‑phase (RP), normal‑phase (NP), and HILIC modes, allowing flexible use without compromising durability or performance.

Application Focus: Diol Chemistry for Polar Compounds

Diol stationary phases are widely chosen for the separation of polar compounds that are difficult to retain in reversed‑phase chromatography. The 40060‑15P column is effective for a broad range of analytes, including:

  • Polar and non‑polar peptides

  • Amino acids

  • Polar metabolites

  • Small biological and biomolecular compounds

Its balanced polarity enables reliable operation across RP, NP, and HILIC methods, making it a very popular choice for new method development in analytical laboratories.

Cogent TYPE‑C™ Silica Hydride Advantages

Compared to traditional bonded‑silica Diol columns, the TYPE‑C™ silica hydride platform provides distinct operational advantages:

  • Faster equilibration, particularly beneficial for HILIC workflows

  • Excellent selectivity for polar and mixed‑polarity analytes

  • Improved robustness and extended column lifetime

  • Reduced susceptibility to silica hydrolysis, supporting long‑term use

These properties make the 40060‑15P well suited for method scouting, optimization, and validation where consistency is critical.

Why Choose the 4.6 mm × 150 mm Format

The 150 mm column length delivers increased resolving power, while the 4.6 mm internal diameter provides higher sample capacity and compatibility with standard analytical HPLC systems. This format is optimized for:

  • Higher resolution separations of complex polar mixtures

  • Improved peak capacity during method development

  • Robust performance in routine analytical and screening workflows

  • Ease of integration with standard detectors and autosamplers

This configuration is ideal when separation confidence and method robustness are prioritized over minimum run time.

Particle Size and Performance

The 4 µm particle size offers a practical balance of efficiency, durability, and manageable backpressure, providing:

  • Consistent peak shape and retention

  • Moderate pressure requirements

  • Broad compatibility with conventional HPLC systems

This makes the column especially suitable for longer gradients commonly used during new method development.

System Requirements and Flow Rates

  • Typical flow range (4.6 mm ID): ~0.8–1.2 mL/min

  • System compatibility: Designed for standard analytical HPLC systems

  • Operational considerations: Flow rates may be adjusted based on mobile‑phase viscosity, temperature, and system pressure limits

No specialized low‑flow pumping hardware is required for this format.

 

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