Prep HPLC Column, Diol, 4um, 100A. 21.2mm ID x 250mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40060‑P21‑250 Diol Preparative HPLC Column is designed for high‑capacity, high‑resolution preparative purification workflows where shorter preparative formats do not provide sufficient separation, peak capacity, or loading tolerance. Manufactured with 4 µm, 100 Å Cogent TYPE‑C™ silica hydride, this column enables efficient isolation and collection of milligram‑to‑gram‑level quantities of polar and amine‑containing compounds while preserving the selectivity and retention behavior fundamental to Diol chromatography.
This format is intended for demanding purification applications involving complex mixtures or closely eluting polar analytes requiring maximum chromatographic resolution.
Preparative Use Case: Why 21.2 mm ID × 250 mm
The 21.2 mm internal diameter classifies this column as full preparative, supporting high‑load purification and large‑volume fraction collection. Compared with semi‑preparative and shorter preparative columns, this format:
Supports very high sample loading
Enables collection of large, clean fractions
Preserves chromatographic selectivity during scale‑up
Supports production‑scale and late‑stage purification workflows
The 250 mm column length delivers the highest resolving power and peak capacity in the Diol preparative series, making it the preferred choice for separating structurally similar polar compounds and complex sample matrices where separation confidence is critical.
Diol TYPE‑C™ Stationary Phase Advantages
Manufactured using Cogent TYPE‑C™ silica hydride technology, the Diol stationary phase provides distinct advantages for preparative HILIC and normal‑phase purification:
Excellent selectivity for polar and amine‑containing compounds
Faster equilibration than conventional bonded‑silica Diol phases
Robust column lifetime, even under high‑organic mobile‑phase conditions
This chemistry is particularly well suited for purification of:
Polar small molecules
Metabolites
Analytes containing amine or basic functional groups
Compounds poorly retained using reversed‑phase chromatography
Made‑to‑Order Columns & Pricing Information
Preparative columns are application‑specific tools and represent a significant investment. To ensure accuracy, compatibility, and performance:
Columns are manufactured to order based on customer requirements
Orders cannot be returned or canceled once placed
Pricing and technical guidance are provided through Customer Service via formal quotation
Please contact Customer Service using our online form or by phone to initiate the quotation process.
System Requirements
Typical operating flow rates: ~10–30 mL/min
Requires a preparative HPLC system capable of:
High‑flow solvent delivery
Large injection volumes
Appropriate detectors and fraction‑collection hardware
Standard analytical and semi‑preparative HPLC systems are not suitable for 21.2 mm ID preparative columns.
Flow rates should be optimized based on mobile‑phase viscosity, system pressure limits, and purification objectives.