Prep HPLC Column, Diol, 4um, 100A. 21.2mm ID x 50mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40060‑P21‑50 Diol Preparative HPLC Column is designed for full preparative purification workflows when semi‑preparative columns no longer provide sufficient loading capacity or collection volume. Manufactured with 4 µm, 100 Å Cogent TYPE‑C™ silica hydride, this column enables efficient isolation and collection of milligram‑to‑gram‑level quantities of polar compounds while preserving the selectivity and retention behavior characteristic of Diol chromatography.
This column is intended for laboratories performing routine or repeated purification of polar and amine‑containing analytes under HILIC, normal‑phase, or mixed‑mode conditions.
Preparative Use Case: Why 21.2 mm ID × 50 mm
The 21.2 mm internal diameter classifies this column as full preparative, making it suitable for high‑load purification workflows where fraction collection and throughput are critical. Compared to 10 mm semi‑preparative columns, this format:
Supports substantially higher sample loading
Enables large‑volume fraction collection
Preserves chromatographic selectivity during scale‑up
Transitions workflows from development to production‑level purification
The 50 mm column length emphasizes throughput and efficiency, enabling rapid separations while maintaining sufficient resolution for targeted purification of polar compounds.
Diol TYPE‑C™ Stationary Phase Advantages
Manufactured using Cogent TYPE‑C™ silica hydride technology, the Diol stationary phase offers distinct advantages for preparative HILIC and normal‑phase purification:
Excellent selectivity for polar and amine‑containing analytes
Faster equilibration than conventional bonded‑silica Diol phases
Robust column lifetime, even under high‑organic mobile‑phase conditions
This chemistry is particularly well suited for purification of:
Polar small molecules
Metabolites
Analytes containing amine or basic functional groups
Compounds with poor retention using reversed‑phase methods
Made‑to‑Order Columns & Pricing Information
Preparative columns represent a significant, application‑specific investment. To ensure correct configuration and system compatibility:
Columns are manufactured to order based on customer requirements
Orders cannot be returned or canceled once placed
Pricing and technical support are provided by Customer Service via formal quotation
Please contact Customer Service using our online form or by phone to initiate the quotation process.
System Requirements
Typical operating flow rates: ~10–30 mL/min
Requires a preparative HPLC system capable of:
High‑flow delivery
Large injection volumes
Suitable detectors and fraction‑collection hardware
Standard analytical and semi‑preparative HPLC systems are not suitable for 21.2 mm ID preparative columns.
Flow rates should be optimized based on mobile‑phase viscosity, pressure limits, and purification objectives.