Catalog Number - 40060-P30-100

The 40060‑P30‑100 Diol Preparative HPLC Column is engineered for high‑capacity, high‑resolution preparative purification workflows where shorter or smaller‑diameter columns no longer provide sufficient resolving power or loading capacity. Manufactured with 4 µm, 100 Å Cogent TYPE‑C™ silica hydride, this column enables efficient isolation and collection of large milligram‑to‑gram‑level quantities of polar and amine‑containing compounds while preserving the selectivity and retention behavior inherent to Diol chromatography.

The increased column length provides improved separation of complex or closely eluting polar analytes while maintaining scalable preparative performance.

Preparative Use Case: Why 30 mm ID × 100 mm

The 30 mm internal diameter classifies this column as large‑scale preparative, supporting substantially higher sample loading and fraction volumes than 21.2 mm formats. Compared to smaller preparative columns, this configuration:

• Supports very high sample loading

• Enables collection of very large fractions

• Preserves chromatographic selectivity during scale‑up

• Supports routine and production‑oriented purification workflows

The 100 mm column length provides increased resolution and peak capacity compared to 50 mm formats, making it well suited for separating more complex polar mixtures while maintaining practical throughput.

Diol TYPE‑C™ Stationary Phase Advantages

Manufactured using Cogent TYPE‑C™ silica hydride technology, the Diol stationary phase offers clear advantages for large‑scale HILIC and normal‑phase purification:

• Excellent selectivity for polar and amine‑containing compounds

• Faster equilibration than conventional bonded‑silica Diol phases

• Robust column lifetime, even under high‑organic mobile‑phase conditions

This chemistry is particularly well suited for purification of:

• Metabolites

• Polar small molecules

• Analytes containing amine or basic functional groups

• Compounds poorly retained using reversed‑phase chromatography

Made‑to‑Order Columns & Pricing Information

Large‑diameter preparative columns are application‑specific tools and represent a significant investment. To ensure proper configuration and compatibility:

• Columns are manufactured to order

• Orders cannot be returned or canceled once placed

• Pricing and technical guidance are provided by Customer Service via formal quotation

• Please contact Customer Service using our online form or by phone to initiate the quotation process.

• View more information on Custom Items.

System Requirements

• Typical operating flow rates: ~20–50 mL/min

• Requires a large‑scale preparative HPLC system capable of:

  • High‑flow solvent delivery

  • Large injection volumes

  • Appropriate detectors and fraction‑collection hardware

Analytical and semi‑preparative HPLC systems are not suitable for 30 mm ID preparative columns.

Flow rates should be optimized based on mobile‑phase viscosity, system pressure limits, and purification objectives.