Prep HPLC Column, Diol, 4um, 100A. 30mm ID x 250mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40060‑P30‑250 Diol Preparative HPLC Column is engineered for high‑capacity, high‑resolution preparative purification workflows where shorter or smaller‑diameter columns no longer provide sufficient separation, peak capacity, or loading tolerance. Manufactured with 4 µm, 100 Å Cogent TYPE‑C™ silica hydride, this column enables efficient isolation and collection of large milligram‑to‑gram‑level quantities of polar and amine‑containing compounds while maintaining the selectivity and retention behavior fundamental to Diol chromatography.
The extended column length provides maximum resolving power, making this column ideal for demanding, late‑stage purification of complex polar mixtures.
Preparative Use Case: Why 30 mm ID × 250 mm
The 30 mm internal diameter classifies this column as large‑scale preparative, enabling very high sample loading and large‑volume fraction collection. Compared with 21.2 mm and shorter 30 mm formats, this configuration:
Supports very high sample loads
Enables collection of large, clean fractions
Preserves chromatographic selectivity during scale‑up
Supports production‑scale and late‑stage purification workflows
The 250 mm column length delivers the highest resolution and peak capacity in the Diol preparative series, making it the preferred choice for separating closely eluting or structurally similar polar compounds where maximum separation confidence is required.
Diol TYPE‑C™ Stationary Phase Advantages
Manufactured using Cogent TYPE‑C™ silica hydride technology, the Diol stationary phase provides distinct advantages for large‑scale HILIC and normal‑phase purification:
Excellent selectivity for polar and amine‑containing compounds
Faster equilibration than conventional bonded‑silica Diol phases
Robust column lifetime, even under extended high‑organic mobile‑phase conditions
This chemistry is particularly well suited for purification of:
Metabolites
Polar small molecules
Analytes containing amine or basic functional groups
Compounds with poor retention using reversed‑phase chromatography
Made‑to‑Order Columns & Pricing Information
Large‑diameter preparative columns are application‑specific tools and represent a significant investment. To ensure correct configuration and compatibility:
Columns are manufactured to order
Orders cannot be returned or canceled once placed
Pricing and technical guidance are provided by Customer Service via formal quotation
Please contact Customer Service using our online form or by phone to initiate the quotation process.
System Requirements
Typical operating flow rates: ~20–50 mL/min
Requires a large‑scale preparative HPLC system capable of:
High‑flow solvent delivery
Very large injection volumes
Industrial‑grade detectors and fraction‑collection systems
Analytical and semi‑preparative HPLC systems are not suitable for 30 mm ID preparative columns.
Flow rates should be optimized based on mobile‑phase viscosity, system pressure limits, and purification objectives.