Prep HPLC Column, Diol, 4um, 100A. 30mm ID x 50mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40060‑P30‑50 Diol Preparative HPLC Column is designed for high‑throughput, high‑capacity preparative purification workflows where 21.2 mm ID columns no longer provide sufficient loading capacity. Manufactured with 4 µm, 100 Å Cogent TYPE‑C™ silica hydride, this column enables efficient isolation and collection of large milligram‑to‑gram‑level quantities of polar and amine‑containing compounds while preserving the selectivity and retention behavior characteristic of Diol chromatography.
This format is intended for preparative laboratories performing routine or repeated purification requiring higher throughput and larger fraction volumes.
Preparative Use Case: Why 30 mm ID × 50 mm
The 30 mm internal diameter classifies this column as large‑scale preparative, enabling substantially higher sample loading and fraction collection than 21.2 mm columns. Compared to smaller preparative formats, this configuration:
Supports very high sample loading
Enables collection of very large fractions
Preserves chromatographic selectivity during scale‑up
Supports production‑oriented purification workflows
The 50 mm column length emphasizes throughput and efficiency, making it ideal for fast preparative isolations where maximum resolution is not required but capacity and speed are critical.
Diol TYPE‑C™ Stationary Phase Advantages
Manufactured using Cogent TYPE‑C™ silica hydride technology, the Diol stationary phase provides clear advantages for large‑scale HILIC and normal‑phase purification:
Excellent selectivity for polar and amine‑containing compounds
Faster equilibration than conventional bonded‑silica Diol phases
Robust column lifetime, even under high‑organic mobile‑phase conditions
This chemistry is particularly effective for purification of:
Metabolites
Polar small molecules
Analytes containing amine or basic functional groups
Compounds with poor retention using reversed‑phase methods
Made‑to‑Order Columns & Pricing Information
Large‑diameter preparative columns are application‑specific tools and represent a significant investment. To ensure proper configuration and compatibility:
Columns are manufactured to order
Orders cannot be returned or canceled once placed
Pricing and technical guidance are provided by Customer Service via formal quotation
Please contact Customer Service using our online form or by phone to initiate the quotation process.
System Requirements
Typical operating flow rates: ~20–50 mL/min
Requires a large‑scale preparative HPLC system capable of:
High‑flow solvent delivery
Very large injection volumes
Industrial‑grade detectors and fraction‑collection systems
Analytical and semi‑preparative HPLC systems are not suitable for 30 mm ID preparative columns.
Flow rates should be optimized based on mobile‑phase viscosity, system pressure limits, and purification objectives.