Semi Prep HPLC Column, Diol, 4um, 100A. 10mm ID x 150mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40060‑SP150 Diol Semi‑Preparative HPLC Column is designed for method scale‑up and sample purification when analytical‑scale columns no longer provide sufficient loading capacity. Manufactured with 4 µm, 100 Å Cogent TYPE‑C™ silica hydride, this column enables efficient isolation and collection of milligram‑level quantities of polar and amine‑containing compounds while preserving the selectivity and retention behavior characteristic of Diol chromatography.
The longer column length provides enhanced resolving power, making this format well suited for complex purification challenges that require increased separation confidence.
Semi‑Preparative Use Case: Why 10 mm ID × 150 mm
The 10 mm internal diameter classifies this column as semi‑preparative, ideal for laboratories transitioning from analytical separations to purification workflows. Compared to standard 4.6 mm analytical columns, this format:
Supports significantly higher sample loading
Enables collection of larger fractions
Preserves chromatographic behavior during method scale‑up
Bridges analytical method development and preparative purification
The 150 mm column length maximizes resolution and peak capacity within the semi‑preparative class, making it especially useful when isolating closely eluting polar compounds or structurally similar analytes.
Diol TYPE‑C™ Stationary Phase Advantages
Manufactured with Cogent TYPE‑C™ silica hydride technology, the Diol stationary phase provides important benefits for semi‑preparative HILIC and normal‑phase purification:
Excellent selectivity for polar and amine‑containing compounds
Faster equilibration than conventional bonded‑silica Diol phases
Robust column lifetime, even under high‑organic mobile‑phase conditions
This chemistry is particularly well suited for purification of:
Metabolites and highly polar small molecules
Analytes containing amine or basic functional groups
Compounds poorly retained by reversed‑phase chromatography
Made‑to‑Order Columns & Pricing Information
These columns are application‑specific purification tools and represent a significant investment. To ensure customers receive the correct column for their workflow:
Columns are produced on order, based on customer requirements
Orders cannot be returned or canceled once placed
Pricing and technical guidance are provided through our Customer Service Department via formal quotation
System Requirements
Typical operating flow rates: ~3–8 mL/min
Requires a semi‑preparative HPLC system capable of:
Higher flow delivery
Increased injection volumes
Appropriate detector and fraction‑collection support
Standard analytical HPLC systems are not suitable for 10 mm ID semi‑preparative columns.
Flow rates may be optimized based on mobile‑phase viscosity, pressure limits, and purification objectives.