Catalog Number - 40060-SP250

The 40060‑SP250 Diol Semi‑Preparative HPLC Column is designed for method scale‑up and high‑resolution sample purification when analytical‑scale columns no longer provide sufficient loading capacity or separation power. Manufactured with 4 µm, 100 Å Cogent TYPE‑C™ silica hydride, this column enables efficient isolation and collection of milligram‑level quantities of polar and amine‑containing compounds while preserving the selectivity and retention characteristics of Diol chromatography.

The extended column length provides maximum resolving power within the semi‑preparative format, making this column ideal for demanding purification challenges involving closely eluting analytes.

Semi‑Preparative Use Case: Why 10 mm ID × 250 mm

The 10 mm internal diameter classifies this column as semi‑preparative, positioning it for laboratories transitioning from analytical methods into purification workflows. Compared to 4.6 mm analytical columns, this format:

• Supports substantially higher sample loading

• Enables collection of larger, cleaner fractions

• Preserves chromatographic behavior during method scale‑up

• Bridges analytical development and preparative purification

The 250 mm column length delivers the highest resolution and peak capacity available in the Diol semi‑prep family, making it especially well suited for isolating structurally similar polar compounds or complex mixtures where maximum separation confidence is required.

Diol TYPE‑C™ Stationary Phase Advantages

Manufactured using Cogent TYPE‑C™ silica hydride technology, the Diol stationary phase provides distinct advantages for semi‑preparative HILIC and normal‑phase purification:

• Excellent selectivity for polar and amine‑containing analytes

• Faster equilibration than conventional bonded‑silica Diol phases

• Robust column lifetime, even under high‑organic mobile‑phase conditions

This chemistry is well suited for purification of:

• Metabolites and highly polar small molecules

• Analytes containing amine or basic functional groups

• Compounds that are poorly retained using reversed‑phase chromatography

Made‑to‑Order Columns & Pricing Information

These columns are application‑specific, made‑to‑order purification tools and represent a significant investment. To ensure proper selection and system compatibility:

• Columns are produced on order, based on customer requirements

• Orders cannot be returned or canceled once placed

• Pricing and technical guidance are provided through our Customer Service Department via formal quotation

Use our online form or contact Customer Service directly to initiate the quotation process.

System Requirements

• Typical operating flow rates: ~3–8 mL/min

• Requires a semi‑preparative HPLC system capable of:

  • Higher flow delivery

  • Increased injection volumes

  • Appropriate detector and fraction‑collection support

Standard analytical HPLC systems are not suitable for 10 mm ID semi‑preparative columns.

Flow rates may be optimized based on mobile‑phase viscosity, pressure limits, and purification objectives.