Semi Prep HPLC Column, Diol, 4um, 100A. 10mm ID x 50mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40060‑SP50 Diol Semi‑Preparative HPLC Column is designed for method scale‑up and sample purification when analytical‑scale columns no longer provide sufficient loading capacity. Built with 4 µm, 100 Å Cogent TYPE‑C™ silica hydride, this column enables efficient isolation and collection of milligram‑level quantities of polar compounds while preserving the selectivity and retention characteristics of Diol chromatography.
This column is well suited for purification workflows where larger injection volumes and fraction collection are required without sacrificing reproducibility or robustness.
Semi‑Preparative Use Case: Why 10 mm ID × 50 mm
The 10 mm internal diameter classifies this column as semi‑preparative, making it ideal for laboratories transitioning from analytical HPLC to purification workflows. Compared to standard 4.6 mm analytical columns, this format:
Supports significantly higher sample loading
Enables collection of larger fractions
Preserves chromatographic behavior during scale‑up
Bridges analytical method development and preparative purification
The 50 mm column length is optimized for throughput and efficiency, allowing rapid separations while maintaining sufficient resolution for targeted isolations of polar and amine‑containing analytes.
Diol TYPE‑C™ Stationary Phase Advantages
Manufactured with Cogent TYPE‑C™ silica hydride technology, the Diol stationary phase provides distinct advantages for semi‑preparative HILIC and normal‑phase separations:
Excellent selectivity for polar and amine‑containing compounds
Faster equilibration than conventional bonded‑silica diol phases
Robust column lifetime, even under high‑organic mobile‑phase conditions
This chemistry is particularly well suited for purification of:
Metabolites and polar small molecules
Analytes containing amine or basic functional groups
Other compounds poorly retained by reversed‑phase methods
Made‑to‑Order Columns & Pricing Information
These columns are application‑specific purification tools and represent a significant investment. To ensure suitability for your workflow:
Columns are produced on order, based on customer requirements
Orders cannot be canceled or returned once placed
Pricing and technical guidance are provided by Customer Service via formal quotation
System Requirements
Typical operating flow rates: ~3–8 mL/min
Requires a semi‑preparative HPLC system capable of:
Higher flow delivery
Increased injection volumes
Appropriate detectors and fraction‑collection support
Standard analytical HPLC systems are not suitable for 10 mm ID semi‑prep columns.
Flow rates may be optimized based on mobile‑phase viscosity, system pressure limits, and purification objectives.