HPLC Column, Silica-C 2.o, 2.2um, 2.1mm ID x 100mm Length, 120A. Cogent TYPE-C Brand. 1 EA..
Additional Info:
The 40200‑10P‑2 Silica‑C™ 2.o HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, delivering near‑UHPLC performance with 2.2 µm particles and 120 Å pore size for high‑pressure analytical separations. The silica hydride surface enables faster equilibration, improved robustness, and enhanced method stability compared to conventional bonded‑silica phases—particularly valuable in HILIC and normal‑phase workflows.
This column is optimized for applications requiring non‑aqueous or low‑water mobile phases, where conventional silica columns may present equilibration and moisture‑control challenges.
Application Focus: Silica‑C™ for HILIC & Normal‑Phase Separations
Silica‑C™ stationary phases are selected for the analysis of compounds that are not soluble in water or require non‑polar or minimally aqueous mobile phases. The Silica‑C™ 2.o phase is particularly effective for:
Lipids
Peptides and proteins
Oligonucleotides
Other compounds requiring non‑polar solvents or HILIC conditions
The TYPE‑C™ silica hydride surface supports stable retention and reproducible performance without the variability often associated with conventional silica.
Key Advantages of Silica‑C™ TYPE‑C™ Technology
Compared to traditional bonded‑silica columns, Silica‑C™ columns provide:
Faster equilibration between injections
No need to control moisture in mobile‑phase solvents
Robust column lifetime under demanding solvent systems
Improved run‑to‑run reproducibility in HILIC and normal‑phase methods
These features make Silica‑C™ a preferred solution for consistent non‑aqueous separations.
Why Choose the 2.1 mm × 100 mm Format
The 2.1 mm ID × 100 mm length configuration is a popular 2.o format when increased resolution is desired, especially for LC‑MS workflows:
Greater resolving power than 50 mm formats
Improved peak capacity for complex samples
Reduced solvent consumption versus larger‑diameter columns
Enhanced LC‑MS compatibility due to lower flow to the MS source
This size balances higher resolution with manageable run times.
Particle Size and Efficiency
The 2.2 µm particle size delivers high efficiency and narrow peak widths under high‑pressure operation, providing near‑UHPLC performance while remaining compatible with many modern analytical HPLC systems.
System Requirements and Flow Rates
Typical flow range (2.1 mm ID): ~0.2–0.3 mL/min
System capability: Requires an HPLC system capable of accurate, stable low‑flow delivery
Important note: Not all standard HPLC systems are suitable for reliable operation at these reduced flow rates
Flow‑rate optimization should consider mobile‑phase viscosity, column temperature, and system pressure limits.