Catalog Number - 40200-15P-2

The 40200‑15P‑2 Silica‑C™ 2.o HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, delivering near‑UHPLC performance with 2.2 µm particles and 120 Å pore size under high‑pressure analytical conditions. The silica hydride surface provides faster equilibration, improved robustness, and enhanced method stability compared to conventional bonded‑silica phases, making it well suited for demanding HILIC and normal‑phase separations.

This column is optimized for applications requiring non‑aqueous or low‑water mobile phases, where traditional silica columns may present equilibration variability or moisture‑control challenges.

Application Focus: Silica‑C™ for HILIC & Normal‑Phase Separations

Silica‑C™ stationary phases are selected for the analysis of compounds that are not soluble in water or require non‑polar or minimally aqueous mobile phases. The Silica‑C™ 2.o phase is particularly effective for:

• Lipids

• Peptides and proteins

• Oligonucleotides

• Other compounds requiring non‑polar solvents or HILIC conditions

The TYPE‑C™ silica hydride surface supports stable retention and reproducible performance without the variability commonly associated with conventional silica phases.

Key Advantages of Silica‑C™ TYPE‑C™ Technology

Compared to traditional bonded‑silica columns, Silica‑C™ columns provide:

• Faster equilibration between injections

• No need to control moisture in mobile‑phase solvents

• Robust column lifetime under demanding solvent systems

• Improved run‑to‑run reproducibility in HILIC and normal‑phase methods

These advantages make Silica‑C™ a preferred solution when consistent, non‑aqueous separations are required.

Why Choose the 2.1 mm × 150 mm Format

The 2.1 mm ID × 150 mm length configuration is selected when maximum resolution is desired, particularly in analytical and LC‑MS workflows:

• Higher resolving power than 50 mm and 100 mm formats

• Increased peak capacity for complex sample mixtures

• Reduced solvent consumption compared to larger‑diameter columns

• Excellent LC‑MS compatibility, with lower solvent flow to the MS source

This format is ideal when separation confidence takes priority over the shortest possible run time.

Particle Size and Efficiency

The 2.2 µm particle size delivers high efficiency and narrow peak widths under high‑pressure operation, enabling improved resolution of closely eluting analytes while remaining compatible with many modern analytical HPLC systems.

LC‑MS‑Focused Performance

The narrow‑bore geometry and stable Silica‑C™ retention behavior support:

• Sharp, symmetrical peak shapes

• Improved signal‑to‑noise ratios

• Reliable quantitative performance across extended gradients

For these reasons, the 40200‑15P‑2 is commonly chosen for LC‑MS and LC‑MS/MS methods requiring enhanced resolution in non‑aqueous separations.

System Requirements and Flow Rates

• Typical flow range (2.1 mm ID): ~0.2–0.3 mL/min

• System capability: Requires an HPLC system capable of accurate, stable low‑flow delivery

• Important note: Not all standard HPLC systems are suitable for reliable operation at these reduced flow rates

Final flow‑rate selection should consider mobile‑phase viscosity, column temperature, and system pressure limits.