Catalog Number - 40231-10P-2

40231-10P-2 Cogent UDA™ (weak cation exchange, WCX) HPLC Column is a highly specialized column built on Cogent TYPE‑C™ silica (silica hydride platform), offering unique mixed‑mode selectivity that extends beyond traditional reversed phase or ion exchange separations.

The UDA™ ligand (Undecanoic Acid) features an 11‑carbon hydrophobic chain with a terminal carboxylic acid group, enabling multiple retention mechanisms:

• Reversed phase (hydrophobic retention)

• Weak cation exchange (ionizable acid functionality)

• HILIC (polar interaction capability)

This combination makes the column exceptionally powerful for resolving polar, basic, ionizable, and structurally similar compounds, particularly in complex matrices where conventional columns struggle.

The TYPE‑C silica base provides critical advantages over traditional silica:

• Faster equilibration

• Reduced dependence on mobile phase water content

• Improved durability and lifetime

100 mm Length – Balanced Resolution for LC‑MS Workflows

The 100 mm length is one of the most widely used formats for analytical separations, offering a balance between speed and resolving power.

• Higher resolution than 50 mm columns

• Improved separation of closely related compounds

• Maintains reasonable run times for routine analysis

✅ Recommended for LC‑MS methods requiring improved resolution without sacrificing throughput

Flow Rate & System Requirements (2.1 mm ID, 2.2 µm)

⚠️ Requires UHPLC or low‑flow capable HPLC system

• Typical flow rate range: ~0.2 – 0.4 mL/min

• Higher backpressure due to 2.2 µm particle size

• Requires precise low‑flow solvent delivery

• Optimized for low‑dispersion systems and small flow cells

✅ Important: Standard HPLC systems must be capable of stable and accurate low‑flow operation

✅ System Fit: Ideal for UHPLC and LC‑MS platforms with low delay volume

Unique Selectivity – Why Choose UDA™ (WCX)

True Mixed‑Mode Retention

• Hydrophobic (RP)

• Ionic (weak cation exchange)

• Polar (HILIC)

Powerful for Complex Matrices

• Resolves co‑eluting compounds

• Effective for ionizable and basic analytes

pH‑Controlled Retention

• WCX functionality active at pH ≥ 6

• Selectivity tunable with pH and ionic strength

✅ Ideal when C18, phenyl, or traditional ion exchange columns do not provide adequate separation

TYPE‑C Silica Advantages

Faster Equilibration

• Reduces method cycle times

No Strict Moisture Control Required

• Easier handling vs traditional HILIC columns

Robust Column Lifetimes

• Durable chemistry for extended use

Low Salt Requirements (HILIC Mode)

• Improved MS compatibility

• Cleaner baselines and reduced contamination

Cogent UDA™ Column Advantages

Multi‑Mode Flexibility

• Single column supports RP, HILIC, and WCX

Near UHPLC Performance (2.2 µm)

• High efficiency with manageable pressure

Optimized for LC‑MS

• Lower salt requirements vs traditional ion exchange

Typical Applications

• LC‑MS method development

• Complex matrix separations

• Polar and ionizable compound analysis

• Basic compound retention and separation

• Pharmaceutical and bioanalytical workflows

Important Usage Notes

• Packed and shipped in reversed‑phase solvents

• Requires UHPLC or low‑flow capable HPLC system

• Optimized for low‑volume systems and high efficiency

• pH control is critical for WCX selectivity