Catalog Number - 69220-05P-2

The 69220‑05P‑2 Phenyl Hydride 2.o HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, combining a phenyl‑selective surface with a silica hydride backbone to deliver excellent selectivity, fast equilibration, and robust column lifetime under high‑pressure conditions. The 2.2 µm particle size provides near‑UHPLC efficiency while remaining compatible with many modern high‑pressure HPLC systems.

This stationary phase is specifically optimized for analytes where π–π interactions and hydrophobic‑aromatic selectivity are required in addition to conventional reversed‑phase or HILIC mechanisms.

Application Focus: Phenyl Hydride Selectivity for Aromatic Compounds

Phenyl Hydride chemistry is chosen when compounds containing aromatic rings are difficult to retain or resolve using standard reversed‑phase columns. The Phenyl Hydride 2.o phase provides enhanced interaction with aromatic systems, supporting improved retention, selectivity, and resolution.

This column is particularly effective for:

• Aromatic amines

• Small peptides with aromatic residues

• Azides and substituted aromatic compounds

• Other analytes exhibiting π–π interaction potential

The TYPE‑C™ silica hydride surface enables faster equilibration and improved stability compared to conventional bonded‑silica phenyl phases.

Why Choose the 2.1 mm × 50 mm Format

The 2.1 mm ID × 50 mm length configuration is optimized for speed, sensitivity, and LC‑MS compatibility:

• Short run times and high sample throughput

• Rapid equilibration between injections

• Reduced solvent consumption and waste

• Lower solvent flow to the MS source, improving ionization efficiency

This format is a favorite for LC‑MS and LC‑MS/MS workflows, especially when aromatic compounds must be resolved quickly and reproducibly.

Particle Size and Efficiency

The 2.2 µm particle size delivers near‑UHPLC‑level efficiency, producing narrow peak widths and high resolution under high‑pressure operation. This provides a strong balance between separation performance and method flexibility on modern analytical HPLC systems.

LC‑MS‑Focused Performance

The reduced internal volume of the 2.1 mm column supports:

• Improved signal‑to‑noise ratios

• Sharp, symmetrical peak shapes

• Reliable quantitative performance

These attributes make the Phenyl Hydride 2.o column well suited for aromatic‑compound analysis by LC‑MS, particularly where selectivity beyond C18 is required.

System Requirements and Flow Rates

• Typical flow range (2.1 mm ID): ~0.2–0.3 mL/min

• System capability: Requires an HPLC system capable of accurate, stable low‑flow delivery

• Important note: Not all standard HPLC systems are suitable for reliable operation at these reduced flow rates

Final flow rates should be optimized based on mobile‑phase viscosity, column temperature, and system pressure limits.