Catalog Number - 69220-05P-3

The 69220‑05P‑3 Phenyl Hydride 2.o HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, combining a phenyl‑selective surface with a silica hydride backbone to deliver excellent selectivity, fast equilibration, and robust column lifetime under high‑pressure analytical conditions. The 2.2 µm particle size provides near‑UHPLC separation efficiency while remaining compatible with standard analytical HPLC systems.

This column is built to order and optimized for applications requiring enhanced aromatic selectivity beyond conventional reversed‑phase materials.

Application Focus: Phenyl Hydride Selectivity for Aromatic Compounds

Phenyl Hydride chemistry is specifically selected for analytes containing aromatic rings that are difficult to retain or resolve using standard reversed‑phase columns. The Phenyl Hydride 2.o phase supports π–π interactions in addition to hydrophobic interactions, delivering improved retention and resolution for aromatic analytes.

This column is particularly effective for:

• Aromatic amines

• Small peptides with aromatic residues

• Azides and substituted aromatic compounds

• Other analytes where phenyl‑based selectivity is advantageous

The TYPE‑C™ silica hydride surface enables faster equilibration and improved stability compared to conventional bonded‑silica phenyl phases.

Why Choose the 3.0 mm × 50 mm Format

The 3.0 mm ID × 50 mm length configuration delivers performance similar to traditional 4.6 mm ID analytical columns while offering several efficiency advantages:

• Comparable run times to 4.6 mm ID columns

• Reduced mobile‑phase consumption

• Lower operating flow rates

• Reduced backpressure compared to smaller‑particle, larger‑ID formats

This format allows laboratories to achieve efficient aromatic separations without requiring specialized low‑flow hardware or UHPLC‑only systems.

Particle Size and Efficiency

The 2.2 µm particle size provides high efficiency and sharp peak shapes under high‑pressure operation. This allows improved resolution of aromatic compounds while maintaining flexibility on standard analytical HPLC platforms.

LC‑MS and Analytical Performance

The reduced solvent usage and stable retention behavior make this column well suited for LC‑MS and LC‑MS/MS analysis of aromatic compounds, providing:

• Symmetrical peak shapes

• Improved sensitivity

• Reliable quantitative repeatability

System Requirements and Flow Rates

• Typical flow range (3.0 mm ID): ~0.4–0.6 mL/min

• System capability: Compatible with standard analytical HPLC systems

• Note: No special low‑flow or UHPLC‑only equipment is required

Final flow rates should be optimized based on mobile‑phase viscosity, temperature, and system pressure limits.

Made‑to‑Order Columns & Pricing Information

This is a custom, made‑to‑order column.

• Columns are manufactured specifically to order

• Orders cannot be canceled once placed

• Custom columns are non‑returnable

• Please contact Customer Service using our online form or by phone to initiate the quotation process.

• View more information on Custom Items.