Catalog Number - 69220-10P-2

The 69220‑10P‑2 Phenyl Hydride 2.o HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, combining a phenyl‑selective surface with a silica hydride backbone to deliver excellent selectivity, fast equilibration, and robust column lifetime under high‑pressure analytical conditions. The 2.2 µm particle size provides near‑UHPLC efficiency, making this column a strong choice for high‑resolution separations of aromatic analytes.

This stationary phase is optimized for applications where phenyl‑based π–π interactions are required to achieve selectivity beyond conventional reversed‑phase materials.

Application Focus: Phenyl Hydride Selectivity for Aromatic Compounds

Phenyl Hydride chemistry is selected for compounds containing aromatic rings that are difficult to retain or resolve using standard reversed‑phase columns. The Phenyl Hydride 2.o phase supports enhanced aromatic retention through π–π and hydrophobic interactions, improving separation performance for challenging analytes.

This column is particularly effective for:

• Aromatic amines

• Small peptides with aromatic residues

• Azides and substituted aromatic compounds

• Other analytes benefiting from phenyl‑specific selectivity

The TYPE‑C™ silica hydride surface enables faster equilibration and improved stability compared to traditional bonded‑silica phenyl phases.

Why Choose the 2.1 mm × 100 mm Format

The 2.1 mm ID × 100 mm length configuration is a popular choice when increased resolution is desired, particularly in LC‑MS workflows:

• Higher resolving power than 50 mm formats

• Improved peak capacity for complex aromatic mixtures

• Reduced solvent consumption compared to larger‑diameter columns

• Enhanced LC‑MS compatibility with lower flow to the MS source

This size is frequently selected for LC‑MS and LC‑MS/MS methods requiring additional separation confidence without excessive run times.

Particle Size and Efficiency

The 2.2 µm particle size delivers high efficiency and narrow peak widths under high‑pressure operation, enabling improved resolution of closely eluting aromatic compounds while remaining compatible with modern analytical HPLC systems.

LC‑MS‑Focused Performance

The reduced internal volume and stable Phenyl Hydride retention behavior support:

• Sharp, symmetrical peak shapes

• Improved signal‑to‑noise ratios

• Reliable quantitative performance

These features make the 69220‑10P‑2 column especially well suited for LC‑MS analysis of aromatic compounds where enhanced selectivity is required.

System Requirements and Flow Rates

• Typical flow range (2.1 mm ID): ~0.2–0.3 mL/min

• System capability: Requires an HPLC system capable of accurate, stable low‑flow delivery

• Important note: Not all standard HPLC systems are suitable for reliable operation at these reduced flow rates

Final flow rates should be optimized based on mobile‑phase viscosity, column temperature, and system pressure limits.