Catalog Number - 69220-10P-3

The 69220‑10P‑3 Phenyl Hydride 2.o HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, combining a phenyl‑selective surface with a silica hydride backbone to deliver excellent selectivity, fast equilibration, and robust column lifetime under high‑pressure analytical conditions. The 2.2 µm particle size provides near‑UHPLC efficiency while remaining compatible with standard analytical HPLC systems.

This column is built to order and optimized for separations where aromatic‑specific selectivity is required beyond conventional reversed‑phase materials.

Application Focus: Phenyl Hydride Selectivity for Aromatic Compounds

Phenyl Hydride chemistry is selected for aromatic compounds that are difficult to retain or resolve on standard reversed‑phase columns. The Phenyl Hydride 2.o phase supports enhanced selectivity through π–π interactions, improving retention and resolution of challenging analytes.

This column is particularly effective for:

• Aromatic amines

• Small peptides with aromatic residues

• Azides and substituted aromatic compounds

• Other analytes requiring phenyl‑based selectivity

The TYPE‑C™ silica hydride surface provides faster equilibration and improved stability compared to conventional bonded‑silica phenyl phases.

Why Choose the 3.0 mm × 100 mm Format

The 3.0 mm ID × 100 mm length configuration delivers performance similar to traditional 4.6 mm ID analytical columns, while offering operational and cost advantages:

• Comparable run times to 4.6 mm ID columns

• Reduced mobile‑phase consumption

• Lower operating flow rates

• Reduced backpressure, despite the smaller particle size

This format allows laboratories to achieve high‑resolution aromatic separations without requiring specialized low‑flow or UHPLC‑only equipment.

Particle Size and Efficiency

The 2.2 µm particle size delivers high efficiency and narrow peak widths under high‑pressure operation, enabling improved resolution of closely eluting aromatic compounds while maintaining flexibility on conventional analytical HPLC platforms.

LC‑MS and Analytical Performance

Reduced solvent usage and stable Phenyl Hydride retention behavior make this column well suited for LC‑MS and LC‑MS/MS workflows involving aromatic compounds, supporting:

• Symmetrical peak shapes

• Improved sensitivity

• Reliable quantitative repeatability

System Requirements and Flow Rates

• Typical flow range (3.0 mm ID): ~0.4–0.6 mL/min

• System capability: Compatible with standard analytical HPLC systems

• Note: No special low‑flow or UHPLC‑only instrumentation is required

Final flow rates should be optimized based on mobile‑phase viscosity, column temperature, and system pressure limits.

Made‑to‑Order Columns & Pricing Information

This is a custom, made‑to‑order column.

• Columns are manufactured specifically to order

• Orders cannot be canceled once placed

• Custom columns are non‑returnable

• Please contact Customer Service using our online form or by phone to initiate the quotation process.

• View more information on Custom Items.