Catalog Number - 69220-15P-2

The 69220‑15P‑2 Phenyl Hydride 2.o HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, combining a phenyl‑selective surface with a silica hydride backbone to provide excellent selectivity, fast equilibration, and robust column lifetime under high‑pressure analytical conditions. The 2.2 µm particle size delivers near‑UHPLC efficiency, making this column well suited for high‑resolution separations of aromatic compounds.

This stationary phase is optimized for applications requiring phenyl‑based π–π interactions to achieve separations that are difficult or impossible with conventional reversed‑phase columns.

Application Focus: Phenyl Hydride Selectivity for Aromatic Compounds

Phenyl Hydride chemistry is selected for analytes containing aromatic rings that are poorly retained or inadequately resolved using standard RP materials. The Phenyl Hydride 2.o phase enhances retention and resolution through π–π and hydrophobic interactions, delivering improved selectivity for challenging compounds.

This column is particularly effective for:

• Aromatic amines

• Small peptides with aromatic residues

• Azides and substituted aromatic compounds

• Other analytes benefiting from phenyl‑specific selectivity

The TYPE‑C™ silica hydride surface enables faster equilibration and improved stability compared to traditional bonded‑silica phenyl phases.

Why Choose the 2.1 mm × 150 mm Format

The 2.1 mm ID × 150 mm length configuration is a very popular analytical format when maximum resolution is desired, particularly in LC‑MS workflows:

• Higher resolving power than 50 mm and 100 mm columns

• Increased peak capacity for complex aromatic mixtures

• Reduced solvent consumption compared to larger‑diameter columns

• Enhanced LC‑MS compatibility due to lower solvent flow into the MS source

This size is frequently chosen when separation confidence is prioritized over shortest run time.

Particle Size and Efficiency

The 2.2 µm particle size provides high efficiency and narrow peak widths under high‑pressure operation, enabling improved resolution of closely eluting aromatic analytes while remaining compatible with most modern analytical HPLC systems.

LC‑MS‑Focused Performance

The reduced internal volume and stable Phenyl Hydride retention behavior support:

• Sharp, symmetrical peak shapes

• Improved signal‑to‑noise ratios

• Reliable quantitative performance across extended gradients

For these reasons, the 69220‑15P‑2 is a preferred choice for LC‑MS and LC‑MS/MS analysis when additional aromatic selectivity and resolution are required.

System Requirements and Flow Rates

• Typical flow range (2.1 mm ID): ~0.2–0.3 mL/min

• System capability: Requires an HPLC system capable of accurate, stable low‑flow delivery

• Important note: Not all standard HPLC systems are suitable for reliable operation at these reduced flow rates

Flow‑rate optimization should consider mobile‑phase viscosity, column temperature, and system pressure limits.