HPLC Column Set, PEEK with a 7.5 mm ID. Includes, a 250 mm x 7.5 mm black, column body tube (5/8-18 threads), plus 2 PEEK 5 um frits, 2 frit spacers & 2 end fittings. ARE-Applied Research brand. 1 EA.
Additional Info:
The 49333‑250 All‑PEEK HPLC Column Set is designed for professional column packers and preparative chromatographers who require maximum loading capacity and high-resolution bioinert separations. The 7.5 mm ID supports increased sample loading, while the 250 mm length delivers extended retention, higher peak capacity, and superior separation efficiency for complex purification workflows.
PEEK (polyether ether ketone) column hardware provides a fully metal‑free flow path, eliminating unwanted interactions with stainless steel surfaces. This is essential for proteins, peptides, nucleic acids, ions, and metal‑sensitive compounds, where adsorption or catalytic activity can negatively impact recovery and chromatographic performance.
✅ Why Choose All‑PEEK Column Hardware
True Bioinert Flow Path Eliminates metal interactions for sensitive analytes
Higher Sample Loading Capacity (7.5 mm ID) Enables semi‑preparative and purification workflows
Maximum Recovery & Peak Integrity Minimizes adsorption and improves reproducibility
Pack Any Stationary Phase Complete flexibility for custom column development
Reusable & Repackable Design Ideal for purification, process development, and scale‑up
Performance & Design
The column body is manufactured using a proprietary extrusion process that produces a smooth, mirror‑like internal surface, supporting:
Uniform, reproducible packing
Reduced turbulence in the flow path
Improved chromatographic performance
Constructed from graphite-filled PEEK, the graphite is incorporated before extrusion, ensuring:
No chemical coatings or surface treatments
Consistent internal surface chemistry
Enhanced durability and long-term usability
Included Components
250 mm × 7.5 mm ID All‑PEEK column body (5/8‑18 threads)
2 × 5 µm PEEK frits
2 × frit spacers
2 × PEEK end fittings
Use Case Positioning
7.5 mm ID / 250 mm Length
Maximum resolution and purification capability
Ideal for complex mixtures and high-capacity separations
Optimized for semi‑preparative purification and method validation
Typical Applications
High-capacity Protein & Peptide Purification
Nucleic Acid Isolation and Characterization
Ion Chromatography of metal-sensitive analytes
Impurity profiling and preparative isolation
Scale‑up of analytical methods to purification workflows
Bioinert LC and LC‑MS applications
⚠️ When to Use PEEK Column Hardware
Choose All‑PEEK columns when:
Analytes are sensitive to stainless steel interaction
Higher sample loading and purification efficiency are required
Working with biomolecules or coordination-sensitive compounds
Packing & Compatibility
For optimal performance:
Use with ARE‑Applied Research™ All‑PEEK slurry reservoir kits
Select frit porosity appropriate for particle size and scale
Maintain a fully bioinert packing and operating environment
Read guidelines for packing All‑PEEK HPLC columns and achieving consistent column performance
Specifications
Hardware Type: All‑PEEK Column Set
Internal Diameter: 7.5 mm
Length: 250 mm
Thread Type: 5/8‑18
Material: Extruded PEEK (graphite‑filled)
Frits: 5 µm PEEK
Additional Components: Frit spacers included
Flow Path: Metal‑free (bioinert)
Surface Treatment: None (no coatings)
Reusability: Repackable
Brand: ARE‑Applied Research™
Unit: 1 Each
Resources & Ordering Options