Catalog Number - 49333-300

The 49333‑300 All‑PEEK HPLC Column Set is designed for professional column packers and preparative chromatographers who require maximum purification capability and high-resolution bioinert separations. The 7.5 mm ID supports significant sample loading, while the 300 mm length delivers extended retention, maximum peak capacity, and the highest separation efficiency within semi‑preparative formats.

PEEK (polyether ether ketone) column hardware provides a completely metal‑free flow path, eliminating interactions that can occur between analytes and stainless steel surfaces. This is critical for proteins, peptides, nucleic acids, ions, and metal‑sensitive compounds, where adsorption or catalytic effects can reduce recovery and compromise analytical accuracy.

✅ Why Choose All‑PEEK Column Hardware

• True Bioinert Flow Path Eliminates metal interaction for sensitive analytes

• Maximum Sample Loading Capacity (7.5 mm ID) Supports semi‑preparative purification and scale‑up workflows

• Maximum Recovery & Peak Fidelity Minimizes adsorption and maintains analyte integrity

• Pack Any Stationary Phase Complete flexibility for custom column development

• Reusable & Repackable Design Ideal for purification, process development, and advanced method validation

Performance & Design

The column body is manufactured using a proprietary extrusion process that produces a smooth, mirror‑like internal surface, enabling:

• Highly uniform and reproducible packing

• Reduced turbulence and flow inconsistencies

• Maximum chromatographic efficiency

Constructed from graphite-filled PEEK, with graphite incorporated prior to extrusion, this design ensures:

• No chemical coatings or surface treatments

• Consistent internal surface chemistry

• Durable black exterior for long-term use

Included Components

• 300 mm × 7.5 mm ID All‑PEEK column body (5/8‑18 threads)

• 2 × 5 µm PEEK frits

• 2 × frit spacers

• 2 × PEEK end fittings

Use Case Positioning

7.5 mm ID / 300 mm Length

• Ultra-high resolution and maximum purification capability

• Ideal for complex mixtures and difficult separations

• Best suited for method validation, impurity isolation, and process-scale workflows

Typical Applications

• High-capacity protein and peptide purification

• Nucleic acid isolation and characterization

• Ion chromatography of metal-sensitive analytes

• Impurity profiling and trace-level separations

• Scale‑up from analytical to preparative chromatography

• Bioinert LC and LC‑MS workflows

⚠️ When to Use PEEK Column Hardware

Choose All‑PEEK columns when:

• Analytes are sensitive to stainless steel interaction

• Maximum purification efficiency, resolution, and recovery are required

• Working with biomolecules or coordination-sensitive compounds

Packing & Compatibility

For optimal performance:

• Use with ARE‑Applied Research™ All‑PEEK slurry reservoir kits

• Select frit porosity appropriate for particle size and scale

• Maintain a fully bioinert packing and operating environment

Read guidelines for packing All‑PEEK HPLC columns and achieving consistent column performance

Specifications

• Hardware Type: All‑PEEK Column Set

• Internal Diameter: 7.5 mm

• Length: 300 mm

• Thread Type: 5/8‑18

• Material: Extruded PEEK (graphite‑filled)

• Frits: 5 µm PEEK

• Additional Components: Frit spacers included

• Flow Path: Metal‑free (bioinert)

• Surface Treatment: None (no coatings)

• Reusability: Repackable

• Brand: ARE‑Applied Research™

• Unit: 1 Each

Resources & Ordering Options

• Order All‑PEEK slurry reservoir kits for column packing

• Read guidelines for packing All‑PEEK HPLC columns and achieving consistent column performance