Catalog Number - 49334-03

The 49334‑03 All‑PEEK HPLC Column Set is designed for professional column packers and LC‑MS practitioners who require complete control over bioinert column construction in a low‑flow, small‑diameter format. This 2.1 mm ID hardware set is ideal for packing custom analytical columns using virtually any stationary phase.

Constructed entirely from PEEK (polyether ether ketone), this column provides a fully metal‑free flow path, helping prevent unwanted interactions between analytes and stainless steel. This is critical for proteins, peptides, nucleic acids, ions, and metal‑sensitive small molecules, where adsorption or catalytic effects can negatively impact recovery and data quality.

✅ Why Choose All‑PEEK Column Hardware

• True Bioinert Flow Path Eliminates metal contact to protect sensitive analytes

• Improved Recovery & Peak Integrity Reduces adsorption and secondary interactions

• Pack Any Stationary Phase Full flexibility for custom separations and method development

• Reusable & Repackable Design Ideal for R&D, LC‑MS methods, and optimization workflows

• Optimized for 2.1 mm Low‑Flow Systems (1/4‑28 Threads) Suitable for LC‑MS and solvent-efficient applications

Performance & Design

The column body is manufactured from extruded PEEK tubing using a proprietary process that produces a smooth, mirror‑like internal surface, helping to:

• Improve packing consistency

• Reduce flow disruption

• Enhance chromatographic efficiency

Unlike coated or chemically treated hardware, this column is:

• Not surface treated

• Not chemically modified

• Inherently bioinert by material composition

A controlled amount of graphite is incorporated into the PEEK before extrusion to produce the black finish, improving durability while maintaining structural integrity.

Included Components

• 1 × 30 mm × 2.1 mm ID PEEK Column Body (1/4‑28 threads)

• 2 × 2 µm PEEK Frits

• 2 × PEEK End Fittings

✔ Complete hardware set for packing one column

Use Case Positioning

2.1 mm ID / 30 mm Length

• Ultra-short column format → Fast screening and rapid method development

• Low-flow design → Ideal for LC‑MS systems

• Reduced solvent consumption → Efficient analytical workflows

Typical Applications

• Protein & peptide analysis

• Nucleic acid separations

• Ion chromatography & metal‑sensitive compounds

• LC‑MS method development

• High-throughput screening workflows

• Custom stationary phase evaluation

⚠️ When to Use PEEK Column Hardware

Choose All‑PEEK columns when:

• Samples may adsorb to or react with metal surfaces

• Maximum recovery and reproducibility are required

• Working with biomolecules or coordination-sensitive analytes

• A fully bioinert LC or LC‑MS system is needed

Packing & Compatibility

For optimal results:

• Use with All‑PEEK Slurry Reservoir Kits sized for short 2.1 mm columns

• Select appropriate frit porosity based on particle size

• Maintain a completely metal‑free packing environment

Specifications

• Hardware Type: All‑PEEK Column Set

• Internal Diameter: 2.1 mm

• Length: 30 mm

• Thread Type: 1/4‑28

• Material: Extruded PEEK (graphite‑filled)

• Frits: 2 µm PEEK

• Flow Path: Metal‑free (bioinert)

• Surface Treatment: None

• Reusability: Repackable

• Brand: ARE‑Applied Research™

• Unit: 1 Each

Resources & Ordering Options

• View All‑PEEK Slurry Reservoir Kits for 2.1 mm Column Packing

• Read Guidelines and Schematics for Packing All‑PEEK HPLC Columns