HPLC Column Set, PEEK with a 2.1 mm ID. Includes, a 100 mm x 2.1 mm black, column body tube (1/4-28 threads), plus 2 PEEK 2 um frits & 2 end fittings. ARE-Applied Research brand. 1 EA.

Additional Info:

The 49334‑100 All‑PEEK HPLC Column Set is designed for professional column packers and LC‑MS users who require maximum control over bioinert column construction in a high-efficiency, low-flow format. This 2.1 mm ID hardware set enables in‑house packing of custom HPLC and LC‑MS columns using virtually any stationary phase.

Constructed entirely from PEEK (polyether ether ketone), this column provides a completely metal‑free flow path, helping eliminate interactions between analytes and stainless steel surfaces. This is critical for proteins, peptides, nucleic acids, ions, and metal‑sensitive small molecules, where adsorption or catalytic effects can compromise recovery and chromatographic performance.

✅ Why Choose All‑PEEK Column Hardware

  • True Bioinert Flow Path Eliminates metal contact to protect sensitive analytes

  • Improved Recovery & Peak Integrity Reduces adsorption and unwanted secondary interactions

  • Pack Any Stationary Phase Full flexibility for custom separations and advanced method development

  • Reusable & Repackable Design Ideal for LC‑MS workflows and method optimization

  • Optimized for 2.1 mm Low‑Flow Systems (1/4‑28 Threads) Supports efficient, solvent-saving analytical methods


Performance & Design

The column body is manufactured from extruded PEEK tubing using a proprietary process that produces a smooth, mirror-like inner surface, helping to:

  • Improve packing consistency

  • Reduce flow disruption and eddy diffusion

  • Enhance chromatographic efficiency and resolution

Unlike coated or treated hardware, this column is:

  • Not chemically modified

  • Not surface coated

  • Inherently bioinert through material composition

A controlled amount of graphite is incorporated into the PEEK before extrusion, creating the black finish that enhances durability while maintaining structural stability.


Included Components

  • 1 × 100 mm × 2.1 mm ID PEEK Column Body (1/4‑28 threads)

  • 2 × 2 µm PEEK Frits

  • 2 × PEEK End Fittings

✔ Complete hardware set for packing one column


Use Case Positioning

2.1 mm ID / 100 mm Length

  • Longer column format → Higher resolution and separation efficiency

  • Low-flow design → Ideal for LC‑MS systems

  • Extended interaction length → Improved peak capacity


Typical Applications

  • Protein & peptide separations

  • Nucleic acid analysis

  • Ion chromatography & metal‑sensitive analytes

  • High-resolution LC‑MS methods

  • Bioanalytical method development and validation

  • Advanced stationary phase evaluation


⚠️ When to Use PEEK Column Hardware

Choose All‑PEEK columns when:

  • Samples may adsorb to or react with metal surfaces

  • High recovery and reproducibility are critical

  • Working with biomolecules or coordination-sensitive analytes

  • A fully bioinert LC or LC‑MS system is required


Packing & Compatibility

For optimal results:

  • Use with All‑PEEK Slurry Reservoir Kits designed for longer 2.1 mm columns

  • Select frit porosity based on particle size

  • Maintain a fully bioinert packing and flow path environment


Specifications

  • Hardware Type: All‑PEEK Column Set

  • Internal Diameter: 2.1 mm

  • Length: 100 mm

  • Thread Type: 1/4‑28

  • Material: Extruded PEEK (graphite‑filled)

  • Frits: 2 µm PEEK

  • Flow Path: Metal‑free (bioinert)

  • Surface Treatment: None

  • Reusability: Repackable

  • Brand: ARE‑Applied Research™

  • Unit: 1 Each


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