Catalog Number - 49334-150

The 49334‑150 All‑PEEK HPLC Column Set is designed for advanced chromatographers and LC‑MS users who require maximum separation efficiency and full control over bioinert column construction. This 2.1 mm ID hardware set enables in‑house packing of high-resolution analytical and LC‑MS columns using virtually any stationary phase.

Constructed entirely from PEEK (polyether ether ketone), this column provides a completely metal‑free flow path, eliminating interactions between analytes and stainless steel surfaces. This is essential for proteins, peptides, nucleic acids, ions, and metal‑sensitive compounds, where adsorption or catalytic effects can compromise recovery and analytical accuracy.

✅ Why Choose All‑PEEK Column Hardware

• True Bioinert Flow Path Eliminates metal contact to protect sensitive analytes

• Maximum Recovery & Peak Integrity Reduces adsorption, tailing, and secondary interactions

• Pack Any Stationary Phase Enables advanced method development and custom separations

• Reusable & Repackable Design Ideal for LC‑MS, research, and method optimization

• Optimized for 2.1 mm Low‑Flow Systems (1/4‑28 Threads) Designed for high-efficiency, solvent-saving workflows

Performance & Design

The column body is manufactured from extruded PEEK tubing using a proprietary process that produces a smooth, mirror‑like internal surface, helping to:

• Improve packing consistency

• Reduce flow disruption and eddy diffusion

• Maximize column efficiency and resolution

Unlike coated or modified column hardware, this product is:

• Not surface treated

• Not chemically modified

• Inherently bioinert by material composition

A controlled amount of graphite is incorporated into the PEEK prior to extrusion, creating the black finish for improved durability and long-term performance.

Included Components

• 1 × 150 mm × 2.1 mm ID PEEK Column Body (1/4‑28 threads)

• 2 × 2 µm PEEK Frits

• 2 × PEEK End Fittings

✔ Complete hardware set for packing one column

Use Case Positioning

2.1 mm ID / 150 mm Length

• Long column format → Maximum resolution and peak capacity

• Low-flow design → Ideal for LC‑MS systems

• Extended interaction length → Superior separation performance

Typical Applications

• High-resolution protein & peptide separations

• Nucleic acid analysis

• Ion chromatography & coordination-sensitive analytes

• Advanced LC‑MS method development

• Bioanalytical and pharmaceutical research

• Complex mixture separations requiring maximum efficiency

⚠️ When to Use PEEK Column Hardware

Choose All‑PEEK columns when:

• Samples may adsorb to or react with metal surfaces

• Maximum recovery and chromatographic integrity are required

• Working with biomolecules or metal-sensitive analytes

• A fully bioinert LC or LC‑MS system is required

Packing & Compatibility

For optimal results:

• Use with All‑PEEK Slurry Reservoir Kits designed for long 2.1 mm columns

• Select frit porosity based on particle size

• Maintain a fully bioinert packing and flow path environment

Specifications

• Hardware Type: All‑PEEK Column Set

• Internal Diameter: 2.1 mm

• Length: 150 mm

• Thread Type: 1/4‑28

• Material: Extruded PEEK (graphite‑filled)

• Frits: 2 µm PEEK

• Flow Path: Metal‑free (bioinert)

• Surface Treatment: None

• Reusability: Repackable

• Brand: ARE‑Applied Research™

• Unit: 1 Each

Resources & Ordering Options

• View All‑PEEK Slurry Reservoir Kits for Long 2.1 mm Column Packing

• Read Guidelines for Packing All‑PEEK HPLC Columns and Achieving Consistent Performance