Catalog Number - 49334-250

The 49334‑250 All‑PEEK HPLC Column Set is designed for advanced chromatographers and LC‑MS specialists who require maximum separation efficiency and complete control over bioinert column construction. This 2.1 mm ID hardware set enables in‑house packing of ultra high-resolution analytical and LC‑MS columns using virtually any stationary phase.

Constructed entirely from PEEK (polyether ether ketone), this column provides a fully metal‑free flow path, eliminating interactions between analytes and stainless steel surfaces. This is essential for proteins, peptides, nucleic acids, ions, and metal‑sensitive compounds, where adsorption or catalytic effects can negatively impact recovery, sensitivity, and reproducibility.

✅ Why Choose All‑PEEK Column Hardware

• True Bioinert Flow Path Eliminates metal contact to protect sensitive analytes

• Maximum Recovery & Peak Integrity Minimizes adsorption, tailing, and secondary interactions

• Pack Any Stationary Phase Enables complete flexibility for advanced separations

• Reusable & Repackable Design Ideal for R&D, LC‑MS, and method optimization

• Optimized for 2.1 mm Low‑Flow Systems (1/4‑28 Threads) Designed for high-efficiency, solvent-saving workflows

Performance & Design

The column body is manufactured from extruded PEEK tubing using a proprietary process that produces a smooth, mirror‑like internal surface, helping to:

• Improve packing consistency

• Reduce flow disruption and dispersion

• Maximize column efficiency and peak capacity

Unlike coated or modified column hardware, this product is:

• Not chemically modified

• Not surface coated

• Inherently bioinert by material composition

A controlled amount of graphite is incorporated into the PEEK prior to extrusion, producing the black finish for enhanced durability and long-term structural stability.

Included Components

• 1 × 250 mm × 2.1 mm ID PEEK Column Body (1/4‑28 threads)

• 2 × 2 µm PEEK Frits

• 2 × PEEK End Fittings

✔ Complete hardware set for packing one column

Use Case Positioning

2.1 mm ID / 250 mm Length

• Extra-long column format → Maximum resolution and peak capacity

• Low-flow design → Ideal for LC‑MS systems

• Extended interaction length → Superior separation of complex mixtures

Typical Applications

• Complex protein & peptide separations

• Nucleic acid analysis

• Ion chromatography & coordination-sensitive analytes

• Advanced LC‑MS method development

• High-resolution bioanalytical workflows

• Separation of complex or closely related compounds

⚠️ When to Use PEEK Column Hardware

Choose All‑PEEK columns when:

• Samples may adsorb to or react with metal surfaces

• Maximum recovery and chromatographic integrity are critical

• Working with biomolecules or metal‑sensitive analytes

• A fully bioinert LC or LC‑MS system is required

Packing & Compatibility

For optimal results:

• Use with All‑PEEK Slurry Reservoir Kits designed for long 2.1 mm columns (>100 mm systems)

• Select frit porosity appropriate for particle size

• Maintain a fully bioinert packing and flow path environment

Specifications

• Hardware Type: All‑PEEK Column Set

• Internal Diameter: 2.1 mm

• Length: 250 mm

• Thread Type: 1/4‑28

• Material: Extruded PEEK (graphite‑filled)

• Frits: 2 µm PEEK

• Flow Path: Metal‑free (bioinert)

• Surface Treatment: None

• Reusability: Repackable

• Brand: ARE‑Applied Research™

• Unit: 1 Each

Resources & Ordering Options

• View All‑PEEK Slurry Reservoir Kits for Long 2.1 mm Column Packing (SR1 systems)

• Read Guidelines for Packing All‑PEEK HPLC Columns and Achieving Consistent Performance