Prep HPLC Column, RP Phenyl, Wide Pore, 10um, 300A. 21.2mm ID x 250mm Length. Cogent Brand. 1 EA.
Additional Information:
Cogent RP Phenyl Wide Pore is MICROSOLV's most popular phenyl stationary phase for reversed-phase HPLC and LC-MS applications involving larger molecules. Built on high-purity silica with a C3-phenyl bonded phase and exhaustive double endcapping, it combines hydrophobic retention with strong π-π interactions to provide selectivity that is often not achievable with traditional C18 or C8 columns. The 300 Å pore structure improves analyte accessibility while maintaining the aromatic selectivity that makes RP Phenyl an effective choice for complex purifications.
68024-P21-2503M is a preparative HPLC column packed with 10 µm RP Phenyl Wide Pore particles featuring a 300 Å pore size in a 21.2 mm ID x 250 mm format. The large internal diameter provides substantial sample loading capacity, while the 250 mm length maximizes resolving power and peak capacity for demanding separations. The larger particle size supports higher flow rates with lower operating backpressure, making this column suitable for purification, process development, and scale-up applications. Typical operating flow rates are approximately 15–40 mL/min depending on mobile phase composition, sample load, and system capabilities.
This column is particularly well suited for purification of larger aromatic compounds, positional isomers, peptides, APIs, tocopherols, flavonoids, polynuclear aromatic compounds, nitro-aromatic compounds, natural products, and related compounds. The scalable RP Phenyl chemistry supports efficient transfer from analytical method development to preparative purification workflows.
Key Benefits
RP Phenyl Wide Pore stationary phase
C3-phenyl bonded chemistry
Strong π-π interaction selectivity
300 Å pore size for larger molecules and peptides
High-purity silica support
Fully double endcapped
Reduced silanol activity
Reduced peak tailing
Excellent peak symmetry
Scalable from analytical to preparative chromatography
Typical Applications
Preparative HPLC purification
Fraction collection
Peptide purification
Positional isomer purification
Aromatic pharmaceutical purification
Active pharmaceutical ingredient (API) purification
Tocopherol purification
Flavonoid purification
Natural product isolation
Process development and scale-up
Specifications
Product Type: Preparative HPLC Column
Stationary Phase: RP Phenyl Wide Pore
Chromatography Mode: Reversed Phase
Bonded Phase: C3-Phenyl
Endcapping: Fully Double Endcapped
Particle Size: 10 µm
Pore Size: 300 Å
Column Dimensions: 21.2 mm ID x 250 mm
Length: 250 mm
Typical Flow Rate: 15–40 mL/min
Brand: Cogent
Additional Resources
Special Order Item Information
Important Notes
Designed for purification and fraction collection workflows
Often selected when C18 or C8 phases do not provide adequate selectivity
Wide pore design improves accessibility for larger molecules and peptides
Extended column length provides maximum resolving power for challenging purifications
Requires a preparative HPLC system capable of higher flow rates and fraction collection