Semi Prep HPLC Column, RP Phenyl, Wide Pore, 10um, 300A. 10mm ID x 50mm Length. Cogent Brand. 1 EA.
Additional Information:
Cogent RP Phenyl Wide Pore is MICROSOLV's most popular phenyl stationary phase for reversed-phase HPLC and LC-MS applications involving larger molecules. Built on high-purity silica with a C3-phenyl bonded phase and exhaustive double endcapping, it combines hydrophobic retention with strong π-π interactions to provide enhanced selectivity for aromatic and structurally related compounds that may not separate sufficiently on traditional C18 or C8 columns.
68024-SP50-3M is a semi-preparative HPLC column packed with 10 µm RP Phenyl Wide Pore particles featuring a 300 Å pore size in a 10 mm ID x 50 mm format. The larger internal diameter provides increased sample loading capacity compared to analytical columns, while the shorter column length is optimized for rapid purification cycles, high-throughput separations, and method development. The 300 Å pore structure improves accessibility for larger molecules and peptides, while the 10 µm particle size supports higher flow rates with lower operating backpressure. Typical operating flow rates are approximately 4–12 mL/min depending on sample load, mobile phase composition, and system capabilities.
This column is particularly effective for purification of peptides, larger aromatic compounds, positional isomers, active pharmaceutical ingredients (APIs), tocopherols, flavonoids, natural products, and related compounds. The RP Phenyl chemistry provides a scalable pathway from analytical method development to semi-preparative purification workflows.
Key Benefits
RP Phenyl Wide Pore stationary phase
C3-phenyl bonded chemistry
Strong π-π interaction selectivity
300 Å pore size for larger molecules and peptides
High-purity silica support
Fully double endcapped
Reduced silanol activity
Reduced peak tailing
Excellent peak symmetry
Scalable from analytical to semi-preparative chromatography
Typical Applications
Semi-preparative HPLC purification
Fraction collection
Peptide purification
Positional isomer purification
Aromatic pharmaceutical purification
Active pharmaceutical ingredient (API) purification
Tocopherol purification
Flavonoid purification
Natural product isolation
Method development and scale-up
Specifications
Product Type: Semi-Preparative HPLC Column
Stationary Phase: RP Phenyl Wide Pore
Chromatography Mode: Reversed Phase
Bonded Phase: C3-Phenyl
Endcapping: Fully Double Endcapped
Particle Size: 10 µm
Pore Size: 300 Å
Column Dimensions: 10 mm ID x 50 mm
Length: 50 mm
Typical Flow Rate: 4–12 mL/min
Brand: Cogent
Additional Resources
Special Order Item Information
Important Notes
Designed for purification and fraction collection workflows
Wide pore design improves accessibility for larger molecules and peptides
Short column length supports rapid purification cycles and high-throughput separations
Increased loading capacity compared to analytical columns
Requires a semi-preparative HPLC system capable of higher flow rates and larger injection volumes