Catalog Number - 40260-05D-2

The 40260‑05D‑2 Diol 2.o™ HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology and packed in a bio‑inert, metal‑free stainless‑steel column with metal‑free frits. This silica hydride–based stationary phase delivers excellent selectivity, very fast equilibration, and robust column lifetime for demanding HILIC and LC‑MS applications.

The metal‑free surface treatment provides permanent protection against analyte interaction with stainless steel hardware—an issue that can negatively impact chromatographic performance and data quality for many compounds. This protection is especially important for:

• Proteins and peptides

• Polar small molecules

• Metal‑sensitive or chelating analytes

By eliminating unwanted metal interactions, the bio‑inert column assembly helps produce more accurate, precise, and reproducible results, particularly in LC‑MS workflows.

Application Focus: Diol Chemistry for HILIC & Metal‑Sensitive Analytes

Diol stationary phases are widely chosen for polar compounds that are difficult to retain in reversed‑phase chromatography. The Diol 2.o™ phase is particularly effective for:

• Amino acids

• Polar metabolites

• Small biological compounds

• Metal‑sensitive analytes requiring inert flow paths

When operated in HILIC mode, this column is considered mass‑spectrometry friendly, supporting MS‑compatible mobile phases while maintaining strong retention and reproducible selectivity.

Cogent TYPE‑C™ Silica Hydride & Metal‑Free Advantages

Compared with conventional bonded‑silica Diol columns, the Diol 2.o™ TYPE‑C™ platform provides significant operational advantages:

• Very fast equilibration, particularly in HILIC gradients

• Improved retention stability versus traditional diol water‑layer–dependent phases

• Reduced analyte loss or distortion caused by metal interactions

• Enhanced method reproducibility for sensitive LC‑MS analyses

The combination of silica hydride chemistry and metal‑free hardware makes this column especially valuable for biologically active, polar, or metal‑reactive compounds.

Why Choose the 2.1 mm × 50 mm Format

This short, narrow‑bore configuration is optimized for speed, sensitivity, and solvent efficiency:

• Short run times and rapid method cycling

• Fast equilibration between injections

• Reduced solvent consumption and waste

• Improved MS ionization efficiency due to lower flow rates

This size is a favorite for LC‑MS and LC‑MS/MS, particularly for screening and targeted analysis of polar compounds.

Particle Size and Efficiency

The 2.2 µm particle size delivers near‑UHPLC efficiency while typically remaining within the pressure limits of many standard HPLC systems. This enables high efficiency and narrow peak widths without the need for dedicated UHPLC hardware.

LC‑MS‑Focused Operation

The reduced internal volume and metal‑free flow path support:

• Sharper peak shapes

• Improved signal‑to‑noise ratios

• Reliable quantitative performance

These attributes make the 40260‑05D‑2 column highly suitable for bioanalytical, metabolomics, and peptide‑focused LC‑MS workflows.

System Requirements and Flow Rates

• Optimal flow range (2.1 mm ID): ~0.2–0.3 mL/min

• System capability: Requires an HPLC system capable of accurate, stable low‑flow delivery

• Important note: Not all standard HPLC pumps or flow cells are suitable for precise operation at these reduced flow rates

Final flow rates should be optimized based on mobile‑phase viscosity, column temperature, and system pressure limits.

Made‑to‑Order Columns & Pricing Information

This is a custom, made‑to‑order column.

• Custom items are manufactured specifically to order

• Orders cannot be canceled once placed

• Custom columns are non‑returnable

• Please contact Customer Service for formal quotation, lead time, and ordering support.

• View more information on what is a Custom Order.