Measuring Pump Flow Accuracy in HPLC - How to
Date: 7-APRIL-2012 Last Updated: 5-JULY-2025
How to Measure Pump Flow Accuracy in HPLC – A Simple Guide
To check the flow accuracy of your HPLC pump at 1.0 mL/min, follow this straightforward method:
- Use a clean, dry 5 mL volumetric flask.
- Use a calibrated stopwatch to time how long it takes to collect 5 mL of mobile phase at the set flow rate.
NOTE: This quick check is a basic verification step and is NOT a substitute for a full operational qualification (OQ), which typically involves testing multiple flow rates. Instead, this method helps confirm that your pump is suitable for performing Performance Qualification (PQ) tests as outlined in the Chemical Solutions PQ Kit™ and HSQ Kit™ protocols.
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