SINCE 1992
 
 
 
Category: How To... Information
KB Home
/
ARTICLES, APPNOTES, FAQ
/
How To... Information
Achieve optimal seals when crimping caps on vials - How to
Read More
Adjusted retention time in HPLC - HPLC Primer
Read More
Analyze phosphorylated compounds with the Cogent Diamond Hydride column - Tips & Suggestions
Read More
Are sparging stones good for ozonating water - FAQ
Read More
Avoid microbial growth in HPLC mobile phases - How to
Read More
Better methods than HILIC in HPLC and LCMS - Tips & Suggestions
Read More
Buffer Hints in Capillary Electrophoresis - How To
Read More
Calculate assay of lisinopril dihydrate based on the anhydrous form - How to
Read More
Calculate extra column volume using the MicroSolv HSQ Kit software - How To
Read More
Calculate the ligand density of a bonded phase of an HPLC column - How To
Read More
Calculating theoretical plates N at half height - How To
Read More
Can I change the filter element in the Cogent Column Filters - FAQ
Read More
Can I use the UV-vis Calibration Kit to qualify detection at 200nm - FAQ
Read More
Can the HSQ Kit be used to measure the LOQ and LOD differences between instruments - FAQ
Read More
Can the L3 Linearity Solution in the HSQ kit be used for UV wavelength accuracy - FAQ
Read More
Capillary conditioning in capillary electrophoresis - How To
Read More
Capillary storage in capillary electrophoresis - How To
Read More
Change the type of methods used from DAD to VWD in the HSQ software - How To
Read More
Changing column ID means changing your gradient - Tips & Suggestions
Read More
Check for high pressure traced to the HPLC pump module - Tips & Suggestions
Read More
Choose an appropriate guard column when using a Cogent TYPE-C HPLC column - How To
Read More
Choose an appropriate re-equilibration or post time for gradient HPLC - How To
Read More
Choosing the correct HPLC column coupler - How To
Read More
Cogent silica hydride HPLC columns by order of hydrophobicity - Tips & Suggestions
Read More
Condition Controlled and Zero EOF capillaries - How to
Read More
Convert HPLC columns between Reversed Phase and Normal Phase - How to
Read More
Converting Cogent TYPE-C columns to normal phase after use in reversed phase - How To
Read More
Cut PEEK tubing using a Clean Cut90 cutter - How to
Read More
Cut stainless steel tubing for HPLC properly - How to
Read More
Determine dwell volume of gradient HPLC systems - How To
Read More
Determine limits of detection LOD and limits of quantification LOQ - How To
Read More
Determine pore volume of an HPLC column using HPLC injections - How To
Read More
Determine the asymmetry factor in HPLC at 10 percent peak height - How To
Read More
Determining HPLC method accuracy - How To
Read More
Determining the precision of an HPLC method - Tips & Suggestions
Read More
Determining wavelength accuracy below 230nm for HPLC instruments - How To
Read More
Difficulties with wavelength accuracy readings while performing a PQ of an HPLC - Tips & Suggestions
Read More
Eliminating shoulder peaks in an HPLC method using Cogent RP C18 columns - Tips & Suggestions
Read More
Establishing HPLC method linearity - Tips & Suggestions
Read More
Factors that affect sensitivity in HPLC - Tech Information
Read More
Guidelines for packing in the PEEK column hardware - Tips & Suggestions
Read More
High pressure PEEK fittings pressure limits - FAQ
Read More
How can I fix split peaks with high back pressure in HPLC analyses - FAQ
Read More
How can I improve accuracy and precision when separating polar compounds by HPLC using Aqueous Normal Phase ANP - FAQ
Read More
How can I minimize bubble formation in limited volume vials when filling - FAQ
Read More
How can I separate capsaicinoids in hot sauce by HPLC - Tips & Suggestions
Read More
How can I separate methacrylic acid and dodecyl benzene sulfonic acid - FAQ
Read More
How can I use the MicroSolv Window Maker to burn a larger window - FAQ
Read More
How can sample concentration have an effect on peak shape in HPLC - FAQ
Read More
How do I connect a guard column to an analytical column - FAQ
Read More
How do I get additional technical assistance - FAQ
Read More
How do I keep my solvents degassed on their way to the HPLC pump - FAQ
Read More
How do I know if a crimped cap on a vial has a proper seal - FAQ
Read More
How do I remove a blockage in my CE capillary - FAQ
Read More
How do I stack multiple ColumnStore column storage cabinet units - FAQ
Read More
How do I validate the Excel spreadsheet that comes with the HSQ Kit - FAQ
Read More
How is pressure inside tubing calculated for HPLC - FAQ
Read More
How tight should an autosampler vial cap be to prevent push through of the septa during injection - FAQ
Read More
How to back flush and wash an HPLC column for reuse - Tips & Suggestions
Read More
How to calibrate UV-vis and HPLC detectors with NIST traceable solutions below 230nm - Tips & Suggestions
Read More
How to change ferrules on Di-Ad HPLC column connectors - Tips & Suggestions
Read More
How to choose a suitable mobile phase additive for negative ionization mode LCMS - Tips & Suggestions
Read More
How to clean sparging stones and In line SS filters - Tips & Suggestions
Read More
How to condition Cogent RP or HPS or Axis HPLC columns for storage - Tips & Suggestions
Read More
How to connect HPLC columns using Di-Ad column connectors - Tips & Suggestions
Read More
How to define custom plate measurements for the U-2D Micro-Sample Management System in the Waters Acquity console software - How to
Read More
How to determine concentration of an impurity in HPLC with the relative response factor RRF - How to
Read More
How to fix a saw tooth pattern in the UV baseline - Tips & Suggestions
Read More
How to get improved retention and peak shape for glutamine glutamic acid and aspartic acid - Tips & Suggestions
Read More
How to handle sparging stones to avoid contamination of mobile phases - Tips & Suggestions
Read More
How to improve LOD or detection limits in HPLC - Tips & Suggestions
Read More
How to increase HPLC UV detector response - Tips & Suggestions
Read More
How to keep HPLC columns free of adsorbed contaminants - How To
Read More
How to minimize HPLC column blockage from most common causes - Tips & Suggestions
Read More
How to prepare 10mM ammonium acetate buffer 90:10 in acetonitrile for HPLC and LCMS - Tips & Suggestions
Read More
How to prepare 16mM ammonium acetate buffer 90:10 in acetonitrile for HPLC - Tips & Suggestions
Read More
How to separate phospholipids with HPLC - Tips & Suggestions
Read More
How to tighten screw caps properly - Tips & Suggestions
Read More
Identify used U-2D inserts for multiple projects or in small sample sets - Tips & Suggestions
Read More
If an analyte is only soluble at basic pH can you use a basic mobile phase for my method - FAQ
Read More
Improve or obtain flat baselines in HPLC methods with a gradient - Tips & Suggestions
Read More
Improving peak shapes in an HPLC method in aqueous normal phase ANP - Tips & Suggestions
Read More
Increasing selectivity and resolution for a xylose and ribose method - Tips & Suggestions
Read More
Integrate a peak with a shoulder - How to
Read More
Interpreting mass spectral data - Primer
Read More
Is the software for the MicroSolv HPLC PQ Kit supplied on CD or can it be downloaded - FAQ
Read More
Know when to discard an outlier data point in HPLC by using the Q Test - How To
Read More
Make an injection water plug in Capillary Electrophoresis - How To
Read More
Make PTFE syringe filters hydrophilic - How to
Read More
Measure signal to noise ratio in an HPLC chromatogram - How to
Read More
Measurements of U-2D Micro-Sample Management System - Tech Information
Read More
Measuring pump flow accuracy in HPLC - How to
Read More
Method development tip for maleic hydrazide - Tips & Suggestions
Read More
Mobile Phase recommendation Cogent Diamond Hydride columns for use with serum samples in Aqueous Normal Phase LCMS - Tips & Suggestions
Read More
Obtain a flat baseline in a gradient method without using blank subtraction - How to
Read More
PEEK slurry reservoir 2.1mm ID column packing hardware information - FAQ
Read More
Perform an Ohms Law Plot - How to
Read More
Pre mix your diluent and mobile phase in sample preparation for HPLC - Tips & Suggestions
Read More
Properly cut a fused silica capillary for CE - How To
Read More
Purge metals from HPLC system using EDTA or medronic acid - How To
Read More
Quaternary amines analysis by LCM and finding the right ion - How To
Read More
Recover an HPLC column that has had ammonium acetate precipitated in it - How To
Read More
Relative response factor RRF and correction factor - How To
Read More
Remove a ferrule that is stuck in an HPLC system or column- How To
Read More
Removing polyimide from capillaries without using heat - How To
Read More
Rescaling graphs in the MicroSolv HPLC PQ Kit Excel template - Tips & Suggestions
Read More
Retention and or peak shape for citric acid has changed compared to data from a previous HPLC run - Tips & Suggestions
Read More
Simplifying getting started with HPLC qualification using the HSQ Kit - How To
Read More
Since Cogent TYPE-C columns can be used in Reversed Phase or Normal Phase what do you suggest to switch these solvent systems which are not miscible - FAQ
Read More
Store and condition Cogent TYPE-C HPLC columns - How To
Read More
Store and maintain Cogent Diamond Hydride HPLC columns - How To
Read More
Storing the HPLC column supplied in the HSQ kit - How To
Read More
Storing the HSQ & PQ Kit test solutions when not in use - How To
Read More
Techniques to prepare a 0.05% solution from a tablet for HPLC injection - How to
Read More
Theoretical plate N estimate for real samples - How to
Read More
Two gradients to use for analysis of hydrophilic and or hydrophobic molecules in the same Aqueous Normal Phase ANP method - Tips & Suggestions
Read More
Type of gradient to use for analysis of hydrophilic and hydrophobic molecules in the same HPLC or LCMS run - Tips & Suggestions
Read More
Use a linear flow rate test to check back pressure on an HPLC column - Tech Information
Read More
Use a low concentration of salt in your mobile phase to avoid ion suppression and build up on your inlet source - Tips & Suggestions
Read More
Use bubble point to filter water through hydrophobic membranes - How to
Read More
Using knitted delay tubes KDTs in mass directed prep chromatography - How To
Read More
Using temperature changes for separations of terpenoids with the Cogent Bidentate C18 and UDC-Cholesterol HPLC columns - Tips & Suggestions
Read More
Using the wavelength calibration solution holmium oxide for HPLC UV detector performance qualification - Tips & Suggestions
Read More
Validation protocol for the PQ kit template - Tips & Suggestions
Read More
What are the maximum changes allowed in a USP method- FAQ
Read More
What are the thread specifications for the PEEK columns - FAQ
Read More
What can cause changing retention times and a peak showing up in my blank with a Silica-C column - FAQ
Read More
What does analytical method validation entail for HPLC and LCMS - Primer
Read More
What is 0.1 % w/v ammonium acetate in terms of molarity - FAQ
Read More
What is the correct thread type and tubing to use with the filter sparging stones - FAQ
Read More
What is the difference between PSI, PSIG and PSIA - FAQ
Read More
What is the mobile phase used in the HSQ Kit and how much is used - FAQ
Read More
What is the pilot length or dimension X depth for end fittings uses to connect HPLC columns - FAQ
Read More
What problems can occur when using a dedicated Reversed Phase HPLC instrument for an Aqueous Normal Phase ANP method for the first time - FAQ
Read More
Where a static mixer for HPLC instruments should be placed - Tips & Suggestions
Read More
Which parameters can be determined with the supplied solutions in the MicroSolv PQ Excel template - FAQ
Read More
Which solution from the HSQ Kit do I use for injector carry over testing - FAQ
Read More
Why am I seeing multiple peaks in HPLC runs of fructose - Tips & Suggestions
Read More
Why do I have higher pressure at a lower flow rate in my HPLC runs - FAQ
Read More

© Copyright 2025. MICROSOLV. All Rights Reserved. Website & Hosting by BlueTone Media