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Chromatography How-To Guides, Procedures & Techniques
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Chromatography How-To Guides, Procedures & Techniques

  • Step‑by‑step guidance for common HPLC and CE tasks, including method setup, troubleshooting, calculations, maintenance, and system optimization. A practical resource to help you perform lab procedures with confidence.

Assembly and Installation Instructions for Cogent Guard Column Holders and Cartridges - Tech Information
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Avoid Contamination of Mobile Phases While Using Sparging Stone - Tips & Suggestions
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Blockage Minimization in HPLC Columns - HPLC Primer
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Blockage Removal in a CE Capillary - Tech Information
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Calculate Ligand Density of a Bonded Phase of an HPLC Column - Tech Information
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Calculate The Internal Volume of a Tube - How to
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Calculating Theoretical Plates N Using Peak Width at Half Height in HPLC - Tech Information
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Can you Make PTFE Syringe Filters Hydrophilic - Tech Information
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Capillary Conditioning Procedures for Reliable Capillary Electrophoresis Performance - Tech Information
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CE Capillary Storage Best Practices for Extended Simplus Capillary Life - Tech Information
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Changing Retention Times and a Spurious Peak with a Silica-C column - Troubleshooting
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Clean Sparging Stones and Inline SS Filters - How To
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Cogent Column Filter and Guard Column Placements - How To
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Concentration of an Impurity in HPLC using the Relative Response Factor RRF - HPLC Primer
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Conditioning Procedure for Zero‑Flow and Controlled‑Flow Capillaries - Tech Information
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Convert HPLC Columns Between Reversed Phase and Normal Phase - How to
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Converting Cogent TYPE-C Columns to Normal Phase after use in Reversed Phase - How To
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Correct LC-MS Ion for Quaternary Amine Analysis - Tech Information
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Custom Plate Measurements for the U-2D Micro-Sample Management System in the Waters Acquity Software - Tech Information
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Cut stainless steel tubing for HPLC properly - Tech Information
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Detector Response Improvement in HPLC UV Detectors - Tech Information
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Determining Accuracy of an HPLC Analytical Method - Tech Information
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Determining Efficiency of HPLC Methods 4 um or 2 um Particles - HPLC Primer
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Determining Gradient Dwell Volume in HPLC Systems - Tech Information
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Determining HPLC Column Pore Volume Using Chromatographic Measurements - Tech Information
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Determining Precision of an HPLC Analytical Method - Tech Information
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Determining wavelength accuracy below 230nm for HPLC instruments - Tech Information
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Diode Array and Variable Wavelength Qualification Using Excel Template - How To
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Estimating Theoretical Plate Count (N) for Real HPLC Samples - Tech Information
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Extra Column Volume calculation using the Chemical Solutions Qualification Kit software - Tech Information
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Flat Baselines in Gradient HPLC Without Blank Subtraction - Tech Information
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Glutamine Glutamic Acid and Aspartic Acid Separation - Tech Information
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Holmium Oxide and Caffeine Standards for UV-Vis and HPLC Detector Qualification Below 230 nm - Tech Information
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Holmium Oxide for HPLC UV Detector Performance Qualification - Tips and Suggestions
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How to Change Ferrules on Di-Ad HPLC Connectors - Tech Information
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How to keep HPLC columns free of adsorbed Preventing Contaminant Buildup and Maintaining HPLC Column Performance - Tech Informationcontaminants - How To
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HPLC and LC-MS Method Validation Fundamentals for Regulated Laboratories - Tech Information
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Improve LOD or Detection Limits in HPLC - Tech Information
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Improving Peak Shape in HILIC Methods - Tech Information
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Improving Retention and Peak Shape Reproducibility for Citric Acid Analysis - Tech Information
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Increasing Selectivity and Resolution for a Xylose and Ribose HPLC Method Resolution - Tips and Suggestions
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Injection Water Plugs in Capillary Electrophoresis CE Principles, Benefits, and Best Practices - Tech Information
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Instructions How to Connect HPLC Columns Using Di-Ad Connectors - Tech Information
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Integrate a peak with a shoulder - How to
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Linear Flow and Pressure Testing to Evaluate HPLC Column Performance - Tech Information
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Lisinopril Assay Results Calculation Using a Dihydrate Reference Standard - Tech Information
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Maintain Degassed Solvents Before Entering an HPLC Pump - Tech Information
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Measuring Pump Flow Accuracy in HPLC - Tech Information
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Methacrylic Acid and Dodecyl Benzene Sulfonic Acid Analysis - Tips and Suggestions
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Microbial Growth Prevention in HPLC Mobile Phases - Tech Information
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Minimize Bubble Formation in Limited Volume Vials When Filling - Tech Information
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Optimal Seals When Crimping Caps on Vials - Tech Information
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Packing Guidance for PEEK Column Hardware - Tips and Suggestions
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Peak Asymmetry Factor Calculation at 10 Percent Peak Height in HPLC - Tech Information
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Phosphorylated Compounds Analyzed with Cogent Diamond Hydride Columns - Tech Information
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Prepare a 16mM Ammonium Acetate Buffer 90:10 in Acetonitrile for HPLC - Tips & Suggestions
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Preparing 10 mM Ammonium Acetate Mobile Phases in High-Acetonitrile HILIC Methods - Tech Information
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Priming a Polyethylene Mobile Phase Inlet Filter - Tech Information
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Proper Preparation of PEEK Tubing Using the Clean-Cut90 Cutter - Tech Information
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Proper Storage Procedures for Cogent RP and Cogent HPS HPLC Columns - Tech Information
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Properly Cutting and Cleaving Fused Silica Capillaries for Capillary Electrophoresis CE - Tech Information
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Purge Metals from HPLC System Using EDTA - How To
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Recovering an HPLC Column After Ammonium Acetate Precipitation - Tech Information
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Removing a Stuck Ferrule from an HPLC System or Column - Tech Information
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Removing Polyimide Coating from CE Capillaries Without Heat - Tech Information
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Removing the Polyimide Coating from Zero-Flow CE Capillaries - Tech Information
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Repair a Blocked Frit in an HPLC column - Tips and Suggestions
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Rescaling Residual Graphs in HPLC Qualification Kit Excel Templates - Tech Information
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Selecting the Appropriate Guard Column for Cogent HPLC Columns - Tech Information
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Sensitivity and Signal-to-Noise Ratio in HPLC - Tech Information
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Separate Phospholipids with HPLC – Practical - Tips and Suggestions
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Separating Hydrophilic and Hydrophobic Compounds in the Same HILIC Method - Tech Information
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Sizes of Detection Windows in CE Capillaries - Tech Information
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Split Peaks and High Back Pressure in HPLC Analyses - Troubleshooting
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Starting Instructions for Chemical Solutions HPLC Qualification Kits - How To
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Storage and Readiness of Qualification Kit HPLC Columns - Tech Information
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Storage Conditioning and Solvent Transition Guidelines for Cogent TYPE-C HPLC Columns - Tech Information
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Storage of HSQ and PQ Qualification Kit Solutions - Tech Information
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Storing a Simplus Brand Bare Fused Silica CE Capillaries - Tech Information
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Storing, Conditioning, and Maintaining Cogent Diamond Hydride HPLC Columns - Tech Information
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Techniques to prepare a 0.05% solution from a tablet for HPLC injection - HPLC Primer
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Temperature Changes for Separations of Terpenoids - Tips and Suggestions
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Tighten Screw Caps Properly - Tech Information
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Tubing Internal Diameter and Its Impact on HPLC Pressure and Performance - Tech Information
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Used vs. Unused U‑2D Inserts Visualization in Multi‑Project - Tech Information
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