What are the typical flow rates recommended by HPLC column companies to start with for various column ID - Primer
April 22, 2012
/
/
Although it will vary from column maker to column manufacturer, and is very dependent on the column length back pressure the following is good rule of thumb for common columns.
| Column Dimensions | Recommended Flow Rate |
| 1.0 | 30-60µL/min |
| 2.1 | 0.1-0.6mL/min |
| 3.0 | 0.3-1.5mL/min |
| 4.6 | 0.8-3.0ml/min |
| 7.8 | 4.0-10mL/min |
