Propantheline Bromide HPLC Methods- AppNote
April 30, 2012
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Orthogonal Methods without Ion-Pairing Agents
Click HERE for Column Ordering Information.
In the USP assay method for Propantheline Bromide tablets, Sodium Dodecyl Sulfate (SDS) is used as an Ion-Pairing Agent in the Mobile Phase. The long alkyl chain of this additive makes it particularly difficult and time consuming to load and remove from the HPLC column.
Is this method, not only is the need for Ion Pairing Agents eliminated but the assay can be performed in either the Reversed Phase (Figure A) or Aqueous Normal Phase mode (Figure B).
The Mobile Phase solvents can be used for many methods using these columns. Both methods illustrate good repeatability, with each run from the five-run overlays shown in a different color.
Columns:
------------------------ - Figure A.
----------------------
---
Figure B.
Temperature:
Flow rate: 1.0 mL / minute
Detection: UV @ 254 nm
Sample:
Note: Propantheline Bromide is a Muscarinic Acetylcholine receptor antagonist which is used for the treatment of ulcers, cramps and spasms of the digestive system, and hyperhidrosis.
Attachment No 132 Orthogonal Methods for Propantheline Bromide pdf 0.3 Mb Download File
Click HERE for Column Ordering Information.
In the USP assay method for Propantheline Bromide tablets, Sodium Dodecyl Sulfate (SDS) is used as an Ion-Pairing Agent in the Mobile Phase. The long alkyl chain of this additive makes it particularly difficult and time consuming to load and remove from the HPLC column.
Is this method, not only is the need for Ion Pairing Agents eliminated but the assay can be performed in either the Reversed Phase (Figure A) or Aqueous Normal Phase mode (Figure B).
The Mobile Phase solvents can be used for many methods using these columns. Both methods illustrate good repeatability, with each run from the five-run overlays shown in a different color.
Peak: Propantheline Bromide
Method ConditionsColumns:
- Fig. A: Cogent Phenyl Hydride™, 4µm, 100Å
- Fig. B: Cogent Diamond Hydride™, 4µm, 100Å
- Fig. A: 69020-7.5P
- Fig. B: 70000.7.5P
- Fig. A: 4.6 x 75 mm
- Fig. B: 4.6 x 75 mm
- A: DI Water / 0.1% Formic Acid
- B: 97% Acetonitrile / 3% DI Water / 0.1% Formic Acid
------------------------ - Figure A.
| Time (minutes) | %B |
|---|---|
| 0 | 10 |
| 4 | 50 |
| 5 | 10 |
| Time (minutes) | %B |
|---|---|
| 0 | 80 |
| 4 | 70 |
| 5 | 80 |
- Fig. A: 35˚C
- Fig. B: 25˚C
Flow rate: 1.0 mL / minute
Detection: UV @ 254 nm
Sample:
- Stock Solution: 1.0 mg Propantheline Bromide Diluted with 1 mL of 50:50 A:B
- Working Solution: 100 µL stock diluted with 900 µL 50:50 Solvent A: Solvent B
Note: Propantheline Bromide is a Muscarinic Acetylcholine receptor antagonist which is used for the treatment of ulcers, cramps and spasms of the digestive system, and hyperhidrosis.
Attachment No 132 Orthogonal Methods for Propantheline Bromide pdf 0.3 Mb Download File