Atropine Determination by HPLC - AppNote
Date: 3-MAY-2012 Last Updated: 31-AUGUST-2026
Rugged & Fast Without Pair Ion Reagents
Ion-pair Chromatography (IPC) is commonly used in order to retain Atropine on ordinary Reversed Phase HPLC Columns. Beside long equilibration times with these columns, IPC often suffers from poor robustness.Using an isocratic elution with flow rate 1mL / minutes gave symmetrical peak for this Tropane Alkaloid. The sensitivity is sufficient for the proper determination of Atropine in plasma after intravenous administration of the drug to hospitalized patients.
This method is also useful for testing for drug poisoning and for stability testing of Atropine solutions during manufacturing. The linear range of detection for Atropine was around 5.0µg / mL with a limit of quantification (LOQ) 10.0µg / mL.
Peak: Atropine (RT = 5.94 min)
Method ConditionsColumn: Cogent UDC-Cholesterol™, 4 µm, 100 Å
Catalog No.: 69069-15P
Dimensions: 4.6 x 150 mm
Mobile Phase:
-- A: DI Water + 0.1% Acetic Acid + 0.005% TFA
-- B: Acetonitrile + 0.1% Acetic Acid + 0.005% TFA
Flow rate: 1 mL / minute
Detection: UV @ 214 nm
Injection vol.: 1 μL
Sample Preparation: Prepared in 50% Solution A / 50% Solution B, concentration 1 mg / mL Sample was filtered through a 0.45 µm Nylon Syringe Filter prior to HPLC-UV injections.
Note: Being potentially deadly, Atropine derives its name from Atropos, one of the three Fates who, according to Greek mythology, chose how a person was to die. Atropine is a core medicine in the World Health Organization’s “Essential Drugs List”, which is a list of minimum medical needs for a basic health care system.
