Paracetamol, Aspirin and Salicylic Acid HPLC Method - AppNote
May 8, 2012
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Analgesic Drugs Analyzed by HPLC
A robust and rapid method was developed for the accurate simultaneous determination of Paracetamol, Aspirin, Caffeine, Benzoic Acid as an internal standard and Salicylic Acid (hydrolysis product of Aspirin). This method is rapid and sensitive and therefore suitable for routine quality control of these drugs in dosage forms and for assay methods in biological fluids.
The major challenge in HPLC has been poor Peak Shape of these small molecules and short Column life. This Method has the advantage of very good Peak shapes, excellent Sensitivity, robust and long Column Life.
Column: Cogent Bidentate™, 4 μm, 100 Å
Catalog No.: 40018-75P
Dimensions: 4.6 x 75 mm
Mobile Phase: Isocratic composition: 78:22 A / B
Detection: UV @ 254 nm
Injection vol.: 2 μL
Sample Preparation: 0.02 mg of Acetaminophen, Caffeine and 0.2 mg of Aspirin, Benzoic Acid and Salicylic Acid were dissolved in 1 mL of 50: 50 A / B Solution.
Notes: Acetaminophen (Paracetamol) is analgesic and antipyretic and with Aspirin (non-steroidal anti-inflammatory agent) they are frequently associated in pharmaceutical formulations against the common cold. Aspirin is used in prophylactic “Aspirin therapy” to reduce the risk of stroke or death in patients with myocardial infarction. The American College of Cardiology (ACC), American Heart Association (AHA), and many clinicians currently recommend low-dose “aspirin therapy”.
Attachment No 107 Paracetamol, Aspirin and Salicylic Acid Standards HPLC Method pdf 0.2 Mb Download File
A robust and rapid method was developed for the accurate simultaneous determination of Paracetamol, Aspirin, Caffeine, Benzoic Acid as an internal standard and Salicylic Acid (hydrolysis product of Aspirin). This method is rapid and sensitive and therefore suitable for routine quality control of these drugs in dosage forms and for assay methods in biological fluids.
The major challenge in HPLC has been poor Peak Shape of these small molecules and short Column life. This Method has the advantage of very good Peak shapes, excellent Sensitivity, robust and long Column Life.
Peaks:
1. Acetaminophen
2. Caffeine
3. Acetylsalicylic Acid
(Aspirin)
4. Benzoic Acid
(internal standard)
5. Salicylic Acid
(degradation product of Aspirin)
Column: Cogent Bidentate™, 4 μm, 100 Å
Catalog No.: 40018-75P
Dimensions: 4.6 x 75 mm
Mobile Phase: Isocratic composition: 78:22 A / B
- A: DI Water / 0.1% Formic Acid
- B: Acetonitrile / 0.1% Formic Acid
Detection: UV @ 254 nm
Injection vol.: 2 μL
Sample Preparation: 0.02 mg of Acetaminophen, Caffeine and 0.2 mg of Aspirin, Benzoic Acid and Salicylic Acid were dissolved in 1 mL of 50: 50 A / B Solution.
Notes: Acetaminophen (Paracetamol) is analgesic and antipyretic and with Aspirin (non-steroidal anti-inflammatory agent) they are frequently associated in pharmaceutical formulations against the common cold. Aspirin is used in prophylactic “Aspirin therapy” to reduce the risk of stroke or death in patients with myocardial infarction. The American College of Cardiology (ACC), American Heart Association (AHA), and many clinicians currently recommend low-dose “aspirin therapy”.
Attachment No 107 Paracetamol, Aspirin and Salicylic Acid Standards HPLC Method pdf 0.2 Mb Download File