Adenosine Nucleotides Analysis - AppNote
May 9, 2012
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Date: 9MAY-2012 Last Updated: 22-AUGUST-2026
ATP & AMP Separated in HILIC ModeThe chromatogram below shows an example of the separation possible with this Method for three Adenosine analytes using a HILIC like Gradient.
Peaks:
1. Adenosine-3’,5’-Cyclic Monophosphate
2. Adenosine 5’-Monophosphate (AMP)
3. Adenosine 5’-Triphosphate (ATP)
Column: Cogent Diamond Hydride™, 4μm, 100Å
Catalog No.: 70000-10P-2
Dimensions: 2.1 x 100 mm
Mobile Phase:
-- A: DI Water / 0.1% Ammonium Formate
-- B: 90% Acetonitrile / 10% DI Water / 0.1% Ammonium Formate
Gradient:
| Time (minutes) | %B |
| 0 | 95 |
| 10 | 70 |
Post Time: 5 minutes
Injection vol.: 2 μL
Flow rate: 0.3 mL / minutes
Detection: UV @ 254 nm
Sample Preparation: 0.3 mg of each Nucleotide in 50% Acetonitrile / DI Water +12% Ammonia
Notes: Nucleotides are important Phosphate- containing compounds that are found in living cells and are associated with a broad array of metabolic and biological processes. They have significant roles in the synthesis of DNA and RNA, are involved in signal transduction pathways, function as coenzymes in biosynthetic pathways and serve as energy reservoirs in biological systems.
