Niacin, Riboflavin, Folic Acid, Pyridoxine, Metformin, Thiamine Analyzed with HPLC - AppNote
January 26, 2014
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Niacin, Riboflavin, Folic Acid, Pyridoxine, Metformin, Thiamine
The Cogent Diol Column is a good addition to the TYPE-C™ Silica line of HPLC stationary phases. Here, a variety of common polar analytes are well-retained and separated.
Column: Cogent Diol™, 4μm, 100Å
Catalog No.: 40060-15P-3
Dimensions: 3.0 x 150mm
Mobile Phase:
— A: DI Water / 0.1% Formic Acid (v/v)
— B: Acetonitrile / 0.1% Formic Acid (v/v)
Gradient:
Post Time: 5 minutes
Flow rate: 0.7 mL/minute
Detection: UV @ 254 nm
Injection vol.: 1μL
Sample Preparation: Mixture of reference standards in diluent of 50 / 50 Solvent A / Solvent B.
t 0 : 0.7 minutes
Note: B and C Vitamins are hydrophilic and therefore may be difficult to retain in Reversed Phase methods. Metformin is a highly polar compound used for treatment of type 2 diabetes.
Attachment No 291 Separation of Polar Test Solutes pdf 0.3 Mb Download File
The Cogent Diol Column is a good addition to the TYPE-C™ Silica line of HPLC stationary phases. Here, a variety of common polar analytes are well-retained and separated.
Peaks:
1. Ascorbic acid
2. Niacin
3. Riboflavin
4. Folic acid
5. Pyridoxine
6. Metformin
7. Thiamine
Column: Cogent Diol™, 4μm, 100Å
Catalog No.: 40060-15P-3
Dimensions: 3.0 x 150mm
Mobile Phase:
— A: DI Water / 0.1% Formic Acid (v/v)
— B: Acetonitrile / 0.1% Formic Acid (v/v)
Gradient:
| Time (minutes) | %B |
|---|---|
| 0 | 95 |
| 3 | 95 |
| 10 | 40 |
| 12 | 40 |
| 13 | 95 |
Flow rate: 0.7 mL/minute
Detection: UV @ 254 nm
Injection vol.: 1μL
Sample Preparation: Mixture of reference standards in diluent of 50 / 50 Solvent A / Solvent B.
t 0 : 0.7 minutes
Note: B and C Vitamins are hydrophilic and therefore may be difficult to retain in Reversed Phase methods. Metformin is a highly polar compound used for treatment of type 2 diabetes.
Attachment No 291 Separation of Polar Test Solutes pdf 0.3 Mb Download File