Hesperidin Spiked Extract Analyzed with LCMS - AppNote
February 7, 2014
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Date: 7-FEBRUARY-2014 Last Updated: 11-AUGUST-2026
Eriocotrin, Hesperidin, Naringin, DiosminCogent™ Phenyl Hydride HPLC Column Specifications, Aromatic Compound Selectivity, Applications, and Ordering Information
In this method, the Hesperidin extract was spiked with three glycosidic flavonoids. The Cogent Phenyl Hydride Column was used to separate the main component of the extract (Hesperidin) and three standards. The MS detection helped to distinguish between the Naringin peak of the standard and a small impurity peak (peak marked “?”). When the Hesperidin extract sample was analyzed, only peaks for Hesperidin and a small peak for Dosmin were detected.
Peaks:
1. Eriocitrin
595.2 [M+H]+,
2. Hesperidin
541.2 m/z [M+H]+,
3. Naringin
581.2 m/z [M+H]+,
4. Diosmin
609.2 m/z [M+H]+
Column: Cogent Phenyl Hydride, 4 µm, 100 Å
Catalog No.: 69020-05P-2
Dimensions: 2.1 x 50 mm
Mobile Phase:
- A: DI Water / 0.1% Formic Acid (v/v)
- B: Acetonitrile / 0.1% Formic Acid (v/v)
Gradient:
| Time (minutes) | %B |
|---|---|
| 0 | 10 |
| 1 | 10 |
| 9 | 70 |
| 10 | 10 |
Post Time: 3 minutes
Injection vol.: 1 µL
Flow rate: 0.4 mL / minute
Detection: ESI – POS - PerkinElmer, Flexar SQ 300 Mass Spectrometer
Samples:
- A: Hesperidin extract (1 mg / mL) in DI Water, diluted 1:100 with Solvent B
- B: Hesperidin extract (1 mg / mL) in DI Water, diluted 1:100 with Solvent B and spiked with 20 ppm of the - standards (Eriocitrin, Naringin, Diosmin)
Note: Flavonoids are phenolic substances isolated from a variety of plants and fruits. They are low molecular weight compounds and in plants they are usually bound to sugar molecules as O-glycosides. Flavonoids are present in foods and beverages of plant origin, such as fruits, vegetables, tea, wine etc. They can be divided into fourteen classes, including flavonols, flavones, anthocyanins, isoflavonoids, and flavanones. They have been shown to have anti-inflammatory, anti-allergic, anti-carcinogenic, antihypertensive and anti-arthritic, and free radical scavenging properties.
Download Application Note: Spiked Hesperidin Extract HPLC Analysis