Atorvastatin HPLC Method Transfer to UHPLC - AppNote
May 22, 2014
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Date: 22-MAY-2014 Last Updated: 31-AUGUST-2026
Transfer to "near-UHPLC" 2.2um Column, for the API Atorvastatin
This Application Note demonstrates how data obtained on a 4μm Cogent Bidentate C18 Column (Figure B) can be adapted for a 2.2μm Column (Figure A). The retention times of both analytes are very comparable in each figure. This Method produces excellent Separation of the API and its main acid degradant and is LCMS compatible which can be used in clinical applications involving plasma samples.
Peaks:
1. Atorvastatin,
2. Atorvastatin Lactone
Columns:
-- Figure A: Cogent Bidentate C18 2.o™, 2.2 μm, 120 Å
-- Figure B: Cogent Bidentate C18™, 4 μm, 100 Å
Catalog Nos.:
-- Figure A: 40218-05P-2
-- Figure B: 40018-05P-2
Column Dimensions: 2.1 x 50 mm
Mobile Phases:
-- A: DI Water / 10m M Ammonium Acetate
-- B: 90:10 Acetonitrile / DI Water / 10m M Ammonium Acetate
Gradients:
| Time (minutes) | %B |
| 0 | 40 |
| 1 | 100 |
| 6 | 100 |
| 7 | 40 |
Detection: UV @ 248 nm
Sample Preparation: 40 mg strength Lipitor® tablet (Atorvastatin) was ground and added to a 50 mL volumetric flask with a portion of Solvent B diluent. The solution was sonicated 10 minutes and diluted to mark with Solvent B. It was then filtered through a 0.4 μm Nylon Syringe Filter. The filtrate was diluted 4x in a diluent of 50:50 Solvent B / 1N HCL. It was heated in a dry bath for 10 minutes at 85°C.
