Tetracycline USP Assay by HPLC- AppNote
April 22, 2021
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USP Method for Tetracycline
This USP Tetracycline Assay is performed and a five injection overlay shows run to run consistency and Precision. The Peak Efficiency is very good and Peak Tailing guidelines are easily met with RSD values less than 0.4%. This demonstrates a good Alternate Column for your USP Method.
Injection vol.: 10 μL
Flow rate: 1.0 mL / minute
Detection: UV @ 280 nm
Sample Preparation: Standard solution: 100 µg / mL of USP Tetracycline in Mobile Phase A
Most recently appeared in Pharmacopeial Forum: Volume No. 43(6) Page Information:
-- USP43-NF38 – 1827
-- USP42-NF37 – 1787
-- USP41-NF36 – 1690
Note: Tetracycline is a broad spectrum antibiotic produced by the Streptomyces genus of Actinobacteria. It exerts a bacteriostatic effect on bacteria by binding reversible to the bacterial 30S ribosomal subunit and blocking incoming aminoacyl tRNA from binding to the ribosome acceptor site. It also binds to some extent to the bacterial 50S ribosomal subunit and may alter the cytoplasmic membrane causing intracellular components to leak from bacterial cells.
This USP Tetracycline Assay is performed and a five injection overlay shows run to run consistency and Precision. The Peak Efficiency is very good and Peak Tailing guidelines are easily met with RSD values less than 0.4%. This demonstrates a good Alternate Column for your USP Method.
Peak:
Tetracycline
Met
hod Conditions:
Column: Cogent RP C18 PE™, 5 μm, 100 Å
Catalog No.: 84518-15P
Dimensions: 4.6 x 150 mm
Mobile Phase:
Column: Cogent RP C18 PE™, 5 μm, 100 Å
Catalog No.: 84518-15P
Dimensions: 4.6 x 150 mm
Mobile Phase:
- A: DI Water 0.1% Phosphoric Acid
- B: Acetonitrile
| Time (minutes) | %B |
|---|---|
| 0 | 15 |
| 7.5 | 15 |
| 7.6 | 40 |
| 10 | 15 |
Injection vol.: 10 μL
Flow rate: 1.0 mL / minute
Detection: UV @ 280 nm
Sample Preparation: Standard solution: 100 µg / mL of USP Tetracycline in Mobile Phase A
Most recently appeared in Pharmacopeial Forum: Volume No. 43(6) Page Information:
-- USP43-NF38 – 1827
-- USP42-NF37 – 1787
-- USP41-NF36 – 1690
Note: Tetracycline is a broad spectrum antibiotic produced by the Streptomyces genus of Actinobacteria. It exerts a bacteriostatic effect on bacteria by binding reversible to the bacterial 30S ribosomal subunit and blocking incoming aminoacyl tRNA from binding to the ribosome acceptor site. It also binds to some extent to the bacterial 50S ribosomal subunit and may alter the cytoplasmic membrane causing intracellular components to leak from bacterial cells.